Plectin-like proteins are present in cells of Chlamydomonas eugametos (Volvocales)
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12503400
DOI
10.1007/bf02818794
Knihovny.cz E-resources
- MeSH
- Algal Proteins metabolism MeSH
- Chlamydomonas metabolism MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Fluorescent Antibody Technique MeSH
- Immunoblotting MeSH
- Rabbits MeSH
- Plectin MeSH
- Intermediate Filament Proteins metabolism MeSH
- Subcellular Fractions metabolism MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Algal Proteins MeSH
- Plectin MeSH
- Intermediate Filament Proteins MeSH
Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green alga Chlamydomonas eugametos (Volvocales). Immunofluorescence and immunoblotting revealed that some of the plectin-like proteins were present in perinuclear region or localized near the cell wall, probably being attached to the cytoplasmic membrane.
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