Enucleolation of porcine oocytes
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12566159
DOI
10.1016/s0093-691x(02)01226-8
PII: S0093691X02012268
Knihovny.cz E-resources
- MeSH
- Cell Division MeSH
- Cell Nucleolus physiology ultrastructure MeSH
- Chromatin ultrastructure MeSH
- Cells, Cultured MeSH
- Metaphase MeSH
- Microsurgery MeSH
- Suction MeSH
- Oocytes physiology ultrastructure MeSH
- Swine * MeSH
- Cell Survival MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chromatin MeSH
Germinal vesicles (GVs) in immature mammalian oocytes contain prominent nucleoli whose role in the process of oocyte maturation is not fully understood. Here we report that the microsurgical removal of nucleoli from immature fully grown porcine oocytes permits germinal vesicle breakdown and chromosome condensation and the enucleolated oocytes mature up to the second metaphase. Interestingly, the enucleolation of growing oocytes which, although unable to mature, resulted in germinal vesicle breakdown and the formation of a cluster of condensed chromatin. These results indicate that the nucleolus in fully grown oocytes is dispensable at least for nuclear maturation. On the other hand, the results obtained in growing oocytes suggest the role of the nucleolus in the cell cycle regulation.
References provided by Crossref.org
Improving the Quality of Oocytes with the Help of Nucleolotransfer Therapy
Nucleoli in embryos: a central structural platform for embryonic chromatin remodeling?
Transplantation of nucleoli into human zygotes: not as simple as expected?
G2/M transition of pig oocytes: How do oocytes initiate maturation?
