DNA homoduplexes containing no pyrimidine nucleotide
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- adenin chemie MeSH
- cirkulární dichroismus * MeSH
- DNA chemie MeSH
- elektroforéza v polyakrylamidovém gelu metody MeSH
- guanin chemie MeSH
- konformace nukleové kyseliny MeSH
- nukleotidy chemie MeSH
- oligonukleotidy chemie MeSH
- párování bází * MeSH
- pyrimidiny chemie MeSH
- tandemové repetitivní sekvence * MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adenin MeSH
- DNA MeSH
- guanin MeSH
- nukleotidy MeSH
- oligonukleotidy MeSH
- pyrimidine MeSH Prohlížeč
- pyrimidiny MeSH
We show using polyacrylamide gel electrophoresis that guanine+adenine repeat strands of DNA associate into homoduplexes at neutral pH and moderate ionic strength. The homoduplexes melt in a cooperative way like the Watson-Crick duplex, although they contain no Watson-Crick base pair. Guanine is absolutely needed for the homoduplex formation and the homoduplex stability increases with the guanine content of the repeat. The present results have implications for the nature of the first replicators, as well as regarding forces stabilizing the duplexes of DNA.
Zobrazit více v PubMed
Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):4922-5 PubMed
Proc Natl Acad Sci U S A. 1987 Jul;84(13):4398-402 PubMed
Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4396-401 PubMed
J Org Chem. 1998;63(26):9652-9656 PubMed
Nucleic Acids Res. 1999 Jan 15;27(2):581-6 PubMed
J Am Chem Soc. 2001 Aug 1;123(30):7439-40 PubMed
EMBO J. 1993 Oct;12(10):4029-38 PubMed
Biochemistry. 2001 Mar 13;40(10 ):3215-21 PubMed
Biopolymers. 2002;67(4-5):275-7 PubMed
Orig Life Evol Biosph. 1995 Jun;25(1-3):83-98 PubMed
Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8158-60 PubMed
J Mol Biol. 1993 Oct 20;233(4):671-81 PubMed
J Mol Biol. 1969 Dec 28;46(3):597-8 PubMed
J Biomol Struct Dyn. 1990 Dec;8(3):491-511 PubMed
J Mol Biol. 1961 Feb;3:71-86 PubMed
Nucleic Acids Res. 1995 Oct 25;23(20):4202-9 PubMed
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1134-5 PubMed
Nucleic Acids Res. 1990 Oct 25;18(20):6057-60 PubMed
EMBO J. 1992 Oct;11(10):3777-86 PubMed
Nature. 1953 May 30;171(4361):964-7 PubMed
Biochemistry. 2000 Oct 24;39(42):12979-88 PubMed
Nat Struct Biol. 1998 Nov;5(11):950-4 PubMed
J Biol Chem. 1997 Apr 4;272(14):9517-23 PubMed
Trends Biochem Sci. 1998 Dec;23(12):491-5 PubMed
J Biol Chem. 1994 Oct 28;269(43):27029-35 PubMed
Prog Nucleic Acid Res Mol Biol. 1998;59:55-94 PubMed
Biopolymers. 2001;62(2):81-4 PubMed
Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7933-8 PubMed
Nucleic Acids Res. 1999 Jan 15;27(2):537-42 PubMed
Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):820-2 PubMed
J Biol Chem. 1996 Aug 23;271(34):20958-64 PubMed
Nucleic Acids Res. 1980 Sep 25;8(18):4305-20 PubMed
Biopolymers. 2000-2001;56(3):123-46 PubMed
Proc Natl Acad Sci U S A. 1962 Dec 15;48:2013-8 PubMed
Biopolymers. 2000-2001;56(3):147-94 PubMed
