DNA homoduplexes containing no pyrimidine nucleotide
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adenine chemistry MeSH
- Circular Dichroism * MeSH
- DNA chemistry MeSH
- Electrophoresis, Polyacrylamide Gel methods MeSH
- Guanine chemistry MeSH
- Nucleic Acid Conformation MeSH
- Nucleotides chemistry MeSH
- Oligonucleotides chemistry MeSH
- Base Pairing * MeSH
- Pyrimidines chemistry MeSH
- Tandem Repeat Sequences * MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenine MeSH
- DNA MeSH
- Guanine MeSH
- Nucleotides MeSH
- Oligonucleotides MeSH
- pyrimidine MeSH Browser
- Pyrimidines MeSH
We show using polyacrylamide gel electrophoresis that guanine+adenine repeat strands of DNA associate into homoduplexes at neutral pH and moderate ionic strength. The homoduplexes melt in a cooperative way like the Watson-Crick duplex, although they contain no Watson-Crick base pair. Guanine is absolutely needed for the homoduplex formation and the homoduplex stability increases with the guanine content of the repeat. The present results have implications for the nature of the first replicators, as well as regarding forces stabilizing the duplexes of DNA.
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