The Role of Alveolar Epithelial Type II-Like Cells in Uptake of Structurally Different Antigens and in Polarisation of Local Immune Responses
Jazyk angličtina Země Spojené státy americké Médium electronic-ecollection
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25894334
PubMed Central
PMC4404363
DOI
10.1371/journal.pone.0124777
PII: PONE-D-14-43937
Knihovny.cz E-zdroje
- MeSH
- alergeny metabolismus MeSH
- alergie MeSH
- antigeny rostlinné chemie MeSH
- antigeny metabolismus MeSH
- cytokiny metabolismus MeSH
- dýchací soustava imunologie MeSH
- epitelové buňky cytologie MeSH
- epitopy T-lymfocytární chemie MeSH
- epitopy chemie MeSH
- fenotyp MeSH
- fluoresceiny chemie MeSH
- imunitní systém MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- plicní alveoly cytologie MeSH
- průtoková cytometrie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 5,6-dicarboxyfluorescein MeSH Prohlížeč
- alergeny MeSH
- antigeny rostlinné MeSH
- antigeny MeSH
- Bet v 1 allergen, Betula MeSH Prohlížeč
- cytokiny MeSH
- epitopy T-lymfocytární MeSH
- epitopy MeSH
- fluoresceiny MeSH
BACKGROUND: Our previous studies on intranasal tolerance induction demonstrated reduction of allergic responses with different allergen constructs. The underlying mechanisms varied depending on their conformation or size. OBJECTIVE: The aim of the present study was to compare the uptake of two structurally different allergen molecules within the respiratory tract following intranasal application. METHODS: The three-dimensional Bet v 1 (Bv1-Protein) and the T cell epitope peptide of Bet v 1 (Bv1-Peptide) were labelled with 5,6-Carboxyfluorescein (FAM) and their uptake was investigated in lung cells and cells of the nasal associated lymphoid tissue from naive and sensitised BALB/c mice. Phenotypic characterisation of FAM+ lung cells after antigen incubation in vitro and after intranasal application was performed by flow cytometry. Impact of Bv1-Protein and Bv1-Peptide on cytokine profiles and gene expression in vivo or in an alveolar epithelial type II (ATII) cell line were assessed in mono- and co-cultures with monocytes using ELISA and quantitative real-time PCR. RESULTS: Both antigens were taken up preferably by ATII-like cells (ATII-LCs) in naive mice, and by macrophages in sensitised mice. After intranasal application, Bv1-Peptide was taken up faster and more efficiently than Bv1-Protein. In vivo and in vitro experiments revealed that Bv1-Protein induced the transcription of thymic stromal lymphopoietin mRNA while Bv1-Peptide induced the transcription of IL-10 and MCP1 mRNA in ATII-LCs. CONCLUSION AND CLINICAL RELEVANCE: Both tested antigens were taken up by ATII-LCs under steady state conditions and induced different polarisation of the immune responses. These data may have an important impact for the generation of novel and more effective prophylactic or therapeutic tools targeting the respiratory mucosa.
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