Cell-cell contacts with epithelial cells modulate the phenotype of human macrophages
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.
Grantová podpora
F05 TW04895
FIC NIH HHS - United States
PubMed
11580100
DOI
10.1023/a:1010975804179
Knihovny.cz E-zdroje
- MeSH
- epitelové buňky imunologie MeSH
- HLA-DR antigeny analýza MeSH
- imunofenotypizace MeSH
- kokultivační techniky MeSH
- ledviny cytologie MeSH
- lidé MeSH
- lipopolysacharidové receptory analýza MeSH
- makrofágový antigen 1 analýza MeSH
- makrofágy imunologie MeSH
- mezibuněčná adhezivní molekula-1 analýza MeSH
- mezibuněčná komunikace imunologie MeSH
- monocyty imunologie MeSH
- nádorové buňky kultivované MeSH
- plíce cytologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- HLA-DR antigeny MeSH
- lipopolysacharidové receptory MeSH
- makrofágový antigen 1 MeSH
- mezibuněčná adhezivní molekula-1 MeSH
Interactions of macrophages with epithelium represent one of the pathways involved in regulating local immune mechanisms. We studied the effect of cell-cell contact with an epithelial monolayer on the phenotype of macrophages. Human monocytes and THP-1 macrophages were co-cultured with monolayers of human bronchial epithelial cells (HBECs), the alveolar type II-like cell line A549, renal adenocarcinoma epithelial cells (RA), and the lung fibroblast strain HFL-1. The expression of CD11b, CD14, CD54, and HLA-DR was measured by immunocytochemistry and flow cytometry and showed epithelial cell induction of CD54 and HLA-DR in monocytes and of all antigens in THP-1 cells. Co-culture with fibroblasts did not change the phenotype of macrophages. Separation by a filter insert inhibited most of the effects. Culture supernatants did not induce prominent phenotypic changes. Cell-cell contacts with epithelium appear to be of importance in regulating the phenotype of macrophages.
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