Nucleoli in large (giant) bi- and multinucleate cells after apoptosis-inducing photodynamic treatment
Language English Country Italy Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12685556
DOI
10.4081/805
Knihovny.cz E-resources
- MeSH
- Apoptosis drug effects MeSH
- Silver Staining MeSH
- Cell Nucleolus chemistry drug effects ultrastructure MeSH
- Photochemotherapy * MeSH
- Photosensitizing Agents pharmacology MeSH
- Granulocytes drug effects pathology MeSH
- HL-60 Cells drug effects pathology MeSH
- Aminolevulinic Acid pharmacology MeSH
- Leukemia drug therapy pathology MeSH
- Humans MeSH
- Giant Cells drug effects ultrastructure MeSH
- Nucleolus Organizer Region chemistry drug effects ultrastructure MeSH
- RNA analysis MeSH
- Light MeSH
- Cell Survival drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Photosensitizing Agents MeSH
- Aminolevulinic Acid MeSH
- RNA MeSH
The present experimental study was undertaken to provide information on nucleolar changes accompanying the apoptotic process in large or giant binucleate and multinucleate cells (LBMNCs). Such cells were present in a small but constant percentage in cultures of HL-60 cells. The apoptotic process was induced by photodynamic treatment (PDT) by means of 5-aminolaevulinic acid (ALA) as the precursor of the photosensitizer protoporphyrin IX and irradiation with broad spectrum blue light (BL). Nucleolar changes in LBMNCs were characterized by marked reduction or disappearance of silver stained particles representing AgNORs in nucleoli including the large ones. In addition, PDT also significantly reduced the number of nucleoli regardless of their size. These changes apparently reflected the decrease or cessation of nucleolar biosynthetic activities and resembled those which were previously observed in naturally maturing bone marrow megakaryocytes (Janoutová et al., 2001).
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