A microprocessor controlled gradient elution system suitable for capillary electrochromatography has been developed and tested. It is based on a liquid handling device described previously which is capable of liquid transport with both low and high fluid dispersion. The low dispersion region formed by stainless steel needle 250 microm I.D. serves for sample injection, while the high dispersion region, created by steep extension of tube diameter, is used for continuous mobile phase gradient generation. A homologous series of seven alkylphenones was electrochromatographically separated on a monolithic polyacrylic column under gradient conditions. An S-shaped acetonitrile gradient (30-70%) was applied. A high reproducibility of retention times (RSD about 0.1%) was obtained, indicating accuracy of automated gradient operations.

Institute of Analytical Chemistry Academy of Sciences of the Czech Republic Veveri 97 61142 Brno Czech Republic

Citace poskytuje Crossref.org

Fractionation of Enriched Phosphopeptides Using pH/Acetonitrile-Gradient-Reversed-Phase Microcolumn Separation in Combination with LC-MS/MS Analysis

Elucidating heterogeneity of IgA1 hinge-region O-glycosylation by use of MALDI-TOF/TOF mass spectrometry: role of cysteine alkylation during sample processing