Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12833166
Knihovny.cz E-resources
- MeSH
- Enzyme Activation drug effects MeSH
- Cytochromes c metabolism MeSH
- Glycerolphosphate Dehydrogenase antagonists & inhibitors metabolism MeSH
- Glycerophosphates metabolism MeSH
- Adipose Tissue, Brown metabolism MeSH
- Cricetinae MeSH
- Mesocricetus MeSH
- Succinic Acid metabolism MeSH
- Oleic Acid antagonists & inhibitors pharmacology MeSH
- Fatty Acids, Nonesterified metabolism MeSH
- Mitochondria drug effects enzymology MeSH
- NAD metabolism MeSH
- NADH Dehydrogenase metabolism MeSH
- Serum Albumin, Bovine pharmacology MeSH
- Cattle MeSH
- Oxygen Consumption drug effects physiology MeSH
- Succinate Cytochrome c Oxidoreductase metabolism MeSH
- Ubiquinone metabolism pharmacology MeSH
- Vitamin K 3 pharmacology MeSH
- Animals MeSH
- Check Tag
- Cricetinae MeSH
- Male MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytochromes c MeSH
- Glycerolphosphate Dehydrogenase MeSH
- Glycerophosphates MeSH
- Succinic Acid MeSH
- Oleic Acid MeSH
- Fatty Acids, Nonesterified MeSH
- NAD MeSH
- NADH Dehydrogenase MeSH
- osteum MeSH Browser
- Serum Albumin, Bovine MeSH
- Succinate Cytochrome c Oxidoreductase MeSH
- Ubiquinone MeSH
- ubiquinone Q3 MeSH Browser
- Vitamin K 3 MeSH
Data presented in this paper show that the size of the endogenous coenzyme Q (CoQ) pool is not a limiting factor in the activation of mitochondrial glycerophosphate-dependent respiration by exogenous CoQ(3), since successive additions of succinate and NADH to brown adipose tissue mitochondria further increase the rate of oxygen uptake. Because the inhibition of glycerophosphate-dependent respiration by oleate was eliminated by added CoQ(3), our data indicate that the activating effect of CoQ(3) is related to the release of the inhibitory effect of endogenous free fatty acids (FFA). Both the inhibitory effect of FFA and the activating effect of CoQ(3) could be demonstrated only for glycerophosphate-dependent respiration, while succinate- or NADH-dependent respiration was not affected. The presented data suggest differences between mitochondrial glycerophosphate dehydrogenase and succinate or NADH dehydrogenases in the transfer of reducing equivalents to the CoQ pool.
