Thirteen unsaturated sterols were identified by gas chromatography--mass spectrometry using serially-coupled capillary columns from the filamentous nitrogen-fixing terrestrial cyanobacterium Scytonema sp. isolated from the microbial community of cyanobacterial on 'Black Cover' biofilms limestone walls in Jerusalem. The dominant sterols were cholest-5-en-3 beta-ol (18.9%), 3 beta-methoxycholest-5-ene (16.2%) and 3 beta-acetoxycholest-5-ene (11.2%).

Institute of Microbiology Academy of Sciences of the Czech Republic 142 20 Prague Czechia

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