Detection of Burkholderia pseudomallei in rice fields with PCR-based technique
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
14533484
DOI
10.1007/bf02931334
Knihovny.cz E-resources
- MeSH
- Burkholderia pseudomallei isolation & purification metabolism MeSH
- DNA, Bacterial isolation & purification MeSH
- Flagellin genetics MeSH
- Polymerase Chain Reaction * MeSH
- Soil Microbiology * MeSH
- DNA, Ribosomal isolation & purification MeSH
- RNA, Ribosomal, 16S isolation & purification MeSH
- Oryza * microbiology MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Taiwan MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Flagellin MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
Burkholderia pseudomallei Ara- in rice fields was detected using PCR-based techniques with 16S RNA and flagella gene primer sets. The sensitivity of these PCRs was at least 1 CFU/mL of B. pseudomallei Ara- preincubated into Ashdown's medium for 6 h. B. pseudomallei Ara- DNA from watery soil were more detectable than from dry soil. The distribution of this DNA was mainly found at a depth of 300-600 mm under crop-covered fields, but not detected in the location of soil close to the land surface. The results suggest that PCR based on 16S RNA and flagella gene primer sets can be applied to investigate the presence of B. pseudomallei Ara- in contaminated soil of rice fields.
See more in PubMed
Folia Microbiol (Praha). 2003;48(2):253-6 PubMed
Southeast Asian J Trop Med Public Health. 1999 Dec;30(4):756-9 PubMed
Int J Epidemiol. 1994 Oct;23(5):1082-90 PubMed
J Infect Dis. 1989 May;159(5):890-9 PubMed
J Med Microbiol. 1996 Dec;45(6):408-12 PubMed
Aust Vet J. 1981 Nov;57(11):535-6 PubMed
Microbiol Immunol. 1999;43(11):995-1001 PubMed
Infect Immun. 1997 Oct;65(10):4319-21 PubMed
Clin Microbiol Rev. 1991 Jan;4(1):52-60 PubMed
J Appl Microbiol. 1997 May;82(5):589-96 PubMed
J Med Microbiol. 1990 Oct;33(2):121-6 PubMed
J Clin Microbiol. 1999 Jun;37(6):1906-12 PubMed
Int J Syst Bacteriol. 1998 Jan;48 Pt 1:317-20 PubMed
Rev Infect Dis. 1989 May-Jun;11(3):413-25 PubMed
Acta Trop. 2000 Feb 5;74(2-3):159-68 PubMed
Southeast Asian J Trop Med Public Health. 1993 Sep;24(3):425-35 PubMed
Am J Trop Med Hyg. 1999 Mar;60(3):458-61 PubMed
Mol Cell Probes. 2002 Jun;16(3):217-22 PubMed
Trans R Soc Trop Med Hyg. 1995 Jan-Feb;89(1):41-3 PubMed
Plant probiotic bacteria: solutions to feed the world
Soil microbial counts and identification of culturable bacteria in an extreme by arid zone
Microbial community structure along an altitude gradient in three different localities
