Detection of Burkholderia pseudomallei in rice fields with PCR-based technique
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
14533484
DOI
10.1007/bf02931334
Knihovny.cz E-zdroje
- MeSH
- Burkholderia pseudomallei izolace a purifikace metabolismus MeSH
- DNA bakterií izolace a purifikace MeSH
- flagelin genetika MeSH
- polymerázová řetězová reakce * MeSH
- půdní mikrobiologie * MeSH
- ribozomální DNA izolace a purifikace MeSH
- RNA ribozomální 16S izolace a purifikace MeSH
- rýže (rod) * mikrobiologie MeSH
- senzitivita a specificita MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Taiwan MeSH
- Názvy látek
- DNA bakterií MeSH
- flagelin MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
Burkholderia pseudomallei Ara- in rice fields was detected using PCR-based techniques with 16S RNA and flagella gene primer sets. The sensitivity of these PCRs was at least 1 CFU/mL of B. pseudomallei Ara- preincubated into Ashdown's medium for 6 h. B. pseudomallei Ara- DNA from watery soil were more detectable than from dry soil. The distribution of this DNA was mainly found at a depth of 300-600 mm under crop-covered fields, but not detected in the location of soil close to the land surface. The results suggest that PCR based on 16S RNA and flagella gene primer sets can be applied to investigate the presence of B. pseudomallei Ara- in contaminated soil of rice fields.
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