A rapid and effective RNA release procedure for virus detection in woody plants by reverse transcription-polymerase chain reaction
Language English Country Switzerland Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
14658842
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Plant Leaves virology MeSH
- Malus virology MeSH
- Plant Diseases virology MeSH
- Reverse Transcriptase Polymerase Chain Reaction methods MeSH
- RNA, Viral isolation & purification MeSH
- RNA Viruses genetics isolation & purification MeSH
- Plant Viruses genetics isolation & purification MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Viral MeSH
A rapid and effective RNA release procedure (RNA-RP) for detection of the Apple stem pitting virus (ASPV) and the Apple stem grooving virus (ASGV) in woody plants by reverse transcription-polymerase chain reaction (RT-PCR) was developed. RNA-RP released RNA into crude homogenate. RNA-RP was compared with classical phenol/chlorophorm extraction of RNA. The RT-PCR detection of ASPV and ASGV was shown to be similar by both the RNA preparation procedures. RNA-RP in contrast to the classical phenol/chloroform procedure represents a reliable and easy-to hand protocol convenient for routine virus detection. Leaf tissues, especially dormant bud leaves and leaves during blossom, were shown to be the most optimal material for detection of these viruses, irrespective of the used RNA preparation procedure.
One-Enzyme RTX-PCR for the Detection of RNA Viruses from Multiple Virus Genera and Crop Plants
