A rapid and effective RNA release procedure for virus detection in woody plants by reverse transcription-polymerase chain reaction
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
14658842
Knihovny.cz E-zdroje
- MeSH
- časové faktory MeSH
- listy rostlin virologie MeSH
- Malus virologie MeSH
- nemoci rostlin virologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí metody MeSH
- RNA virová izolace a purifikace MeSH
- RNA-viry genetika izolace a purifikace MeSH
- rostlinné viry genetika izolace a purifikace MeSH
- senzitivita a specificita MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- RNA virová MeSH
A rapid and effective RNA release procedure (RNA-RP) for detection of the Apple stem pitting virus (ASPV) and the Apple stem grooving virus (ASGV) in woody plants by reverse transcription-polymerase chain reaction (RT-PCR) was developed. RNA-RP released RNA into crude homogenate. RNA-RP was compared with classical phenol/chlorophorm extraction of RNA. The RT-PCR detection of ASPV and ASGV was shown to be similar by both the RNA preparation procedures. RNA-RP in contrast to the classical phenol/chloroform procedure represents a reliable and easy-to hand protocol convenient for routine virus detection. Leaf tissues, especially dormant bud leaves and leaves during blossom, were shown to be the most optimal material for detection of these viruses, irrespective of the used RNA preparation procedure.
One-Enzyme RTX-PCR for the Detection of RNA Viruses from Multiple Virus Genera and Crop Plants
