UVC-protective effect of caffeic acid on normal and transformed human skin cells in vitro
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
14680294
Knihovny.cz E-zdroje
- MeSH
- buněčné dělení účinky léků účinky záření MeSH
- buněčné linie MeSH
- kůže cytologie účinky léků patologie účinky záření MeSH
- kyseliny kávové farmakologie MeSH
- lidé MeSH
- nádorová transformace buněk účinky léků patologie účinky záření MeSH
- ochranné látky farmakologie MeSH
- ultrafialové záření * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- caffeic acid MeSH Prohlížeč
- kyseliny kávové MeSH
- ochranné látky MeSH
Possible UVC-protective properties of CA, a plant phenolic compound with antioxidant activity, were investigated on human KF1 diploid fibroblast and A431 epidermoid carcinoma cell lines. Cell populations, untreated and treated by antioxidants (CA and alpha-tocopherol), were irradiated by UVC at the wavelength of 254 nm and their proliferation activity was determined by the MTT assay. The results show a strong protective effect of CA at both concentrations used (55.5 and 166.5 microM): a significant increase of proliferation activity after UVC irradiation was detected in both cell populations growing in the presence of CA in comparison with cells in DMEM only. The described protective effect of CA was more obvious in transformed cells than in normal diploid cells. This protective ability is probably based on the antioxidant and scavenging activities of CA, which seems to be more efficient than alpha-tocopherol in protection against the cytotoxic effect caused by UVC irradiation.
