Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- kořeny rostlin účinky léků růst a vývoj MeSH
- kultivační média MeSH
- kyselina abscisová farmakologie MeSH
- polyethylenglykoly farmakologie MeSH
- regenerace MeSH
- semena rostlinná účinky léků růst a vývoj ultrastruktura MeSH
- ženšen účinky léků embryologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kultivační média MeSH
- kyselina abscisová MeSH
- polyethylenglykoly MeSH
Embryogenic culture was initiated from mature zygotic embryos of Panax ginseng. Multiple somatic embryos formed and proliferated on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (2.26 microM) and kinetin (0.046 microM). Mature as well as immature somatic embryos grew into plantlets lacking roots on the same media. Histomorphological analysis of somatic embryos treated with abscisic acid (ABA) and polyethylene glycol (PEG 4000) showed a slight improvement in the root meristem organization of torpedo-stage embryos (embryos were more compact and their cells exhibited a lower degree of vacuolation). Shoot regeneration of non-treated somatic embryos was 31% while that for somatic embryos treated with PEG 4000 and ABA was 70%. Moreover, 75% of plants regenerated from PEG- and ABA-treated embryos formed roots while plants from non-treated embryos did not form roots.
