Examination of membrane protein expression in Paracoccus denitrificans by two-dimensional gel electrophoresis
Journal of basic microbiology.
2004 ;
44 (1) :
17-22.
Language English Country Germany Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/pmid14768023
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Aerobiosis MeSH
- Anaerobiosis MeSH
- Genes, Bacterial MeSH
- Bacterial Proteins biosynthesis chemistry genetics isolation & purification MeSH
- Gene Expression * MeSH
- Oxygen metabolism MeSH
- Membrane Proteins biosynthesis chemistry genetics isolation & purification MeSH
- Paracoccus denitrificans genetics growth & development metabolism MeSH
- Periplasm MeSH
- Proteomics MeSH
- Gene Expression Regulation, Bacterial MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Oxygen MeSH
- Membrane Proteins MeSH
The well-known metabolic versatility of the soil bacterium Paracoccus denitrificans poses a challenge for modern proteomic approaches. We describe here improved preparation conditions that allow good separation and quantitative analyses of hundreds of membrane or periplasmic proteins. To illustrate this optimized procedure, the results of a screening for membrane proteins associated predominantly with aerobic or anaerobic (denitrifying) modes of growth are presented.
References provided by Crossref.org
Folia microbiologica.
2011 Jan ;
56 (1) :
13-7.
[epub] 20110312
