Salmonella enterica subsp. enterica serovar Enteritidis harbours ColE1, ColE2, and rolling-circle-like replicating plasmids
Language English Country Canada Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15052312
DOI
10.1139/w03-113
PII: w03-113
Knihovny.cz E-resources
- MeSH
- Bacteriocin Plasmids MeSH
- Nucleic Acid Denaturation MeSH
- DNA, Bacterial analysis chemistry genetics isolation & purification MeSH
- Single-Strand Specific DNA and RNA Endonucleases metabolism MeSH
- Extrachromosomal Inheritance MeSH
- Nucleic Acid Hybridization MeSH
- DNA, Single-Stranded MeSH
- Plasmids * MeSH
- DNA Replication MeSH
- Salmonella enteritidis genetics MeSH
- Sequence Analysis, DNA MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Single-Strand Specific DNA and RNA Endonucleases MeSH
- DNA, Single-Stranded MeSH
Using DNA hybridization, at least three distinct groups of low molecular mass plasmids were identified in Salmonella enterica subsp. enterica serovar Enteritidis. After sequencing representative plasmids from each group, we concluded that they belonged to ColE1, ColE2, and rolling-circle-like replicating plasmids. Plasmid pK (4245 bp) is a representative of widely distributed ColE1 plasmids. Plasmid pP (4301 bp) is homologous to ColE2 plasmids and was present predominantly in single-stranded DNA form. The smallest plasmids pJ (2096 bp) and pB (1983 bp) were classified as rolling-circle-like replicating plasmids. Both encoded only a single protein essential for their own replication, and they must have existed in an unusual molecular structure, as (i) they were capable of hybridization without denaturation, (ii) their DNA could be linearized with S1 nuclease, and (iii) even after such treatment, the ability to hybridize without denaturation did not disappear.
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