Study of Mycobacterium avium complex strains isolated from cattle in the Czech Republic between 1996 and 2000
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15066726
DOI
10.1016/j.vetmic.2004.01.008
PII: S0378113504000112
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial chemistry genetics MeSH
- Mycobacterium avium-intracellulare Infection microbiology veterinary MeSH
- Chickens MeSH
- Mycobacterium avium Complex genetics growth & development isolation & purification MeSH
- Cattle Diseases microbiology MeSH
- Polymerase Chain Reaction veterinary MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Cattle MeSH
- DNA Transposable Elements genetics MeSH
- Tuberculosis microbiology veterinary MeSH
- Age Factors MeSH
- Virulence MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA Transposable Elements MeSH
This study surveys 2,593,348 cattle slaughtered between 1996 and 2000, and further investigates 571 (0.02%) animals found to have tuberculous lesions. Culture of 346 randomly selected tissue samples from animals younger (n = 215) and older (n = 131) than 2 years, isolated mycobacteria from 91 animals (26.3%). These included 74 Mycobacterium avium subsp. avium isolates of IS901+ and IS1245+ genotype and serotype 2, 13M. avium subsp. hominissuis isolates of IS901- and IS1245+ genotype and serotypes 8 (n = 7) and 4 (n = 6), two M. chelonae, one M. avium subsp. paratuberculosis (RFLP type B-C1), and one M. terrae. Culture of mesenteric lymph node samples obtained 66 isolates of M. avium complex (MAC) and four isolates of other mycobacterial species. M. bovis was significantly absent from all samples. Mycobacteria were more frequently (P = 0.01) isolated from tissues of animals under 2 years (34.4%) than animals over 2 years (13.0%). IS901 and IS1245 RFLP methods were used to type 17 randomly selected MAC isolates, virulent after intramuscular inoculation of pullets, from 17 different cattle herds. These revealed 11 distinct IS901 RFLP types and three IS1245 RFLP profiles. Polyclonal infection of individual animals was detected by IS901/IS1245 typing in 2 of the 17 selected isolates.
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