Voltammetry of osmium-modified DNA at a mercury film electrode: application in detecting DNA hybridization
Language English Country Netherlands Media print
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't, Validation Study
PubMed
15110280
DOI
10.1016/j.bioelechem.2003.11.005
PII: S1567539404000428
Knihovny.cz E-resources
- MeSH
- 2,2'-Dipyridyl analogs & derivatives analysis chemistry MeSH
- Staining and Labeling methods MeSH
- Coated Materials, Biocompatible chemistry MeSH
- Biosensing Techniques methods MeSH
- DNA Probes chemistry MeSH
- DNA analysis chemistry MeSH
- Electrochemistry methods MeSH
- Electrodes * MeSH
- Nucleic Acid Hybridization methods MeSH
- Organometallic Compounds analysis chemistry MeSH
- Mercury chemistry MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Validation Study MeSH
- Names of Substances
- 2,2'-Dipyridyl MeSH
- Coated Materials, Biocompatible MeSH
- calf thymus DNA MeSH Browser
- DNA Probes MeSH
- DNA MeSH
- Organometallic Compounds MeSH
- osmium tetroxide-2,2'-bipyridine MeSH Browser
- Mercury MeSH
Mercury film electrodes (MFE) have recently been used in nucleic acid electrochemical analysis as alternatives to the classical mercury drop ones. DNA modified with osmium tetroxide, 2,2'-bipyridine (Os,bipy) can be detected with a high sensitivity at mercury electrodes via measurements of a catalytic osmium signal. In this paper we show that mercury film on a glassy carbon electrode can be used in voltammetric analysis of Os,bipy-modified DNA. Application of the MFE as a detection electrode in double-surface electrochemical DNA hybridization assay involving osmium labeling of target DNA is demonstrated.
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