Chitosanase activity in Bacillus thuringiensis
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15114873
DOI
10.1007/bf02931653
Knihovny.cz E-resources
- MeSH
- Bacillus thuringiensis enzymology MeSH
- Pest Control, Biological MeSH
- Chitin analogs & derivatives metabolism MeSH
- Chitosan MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Chromatography, Gel MeSH
- Glycoside Hydrolases isolation & purification metabolism MeSH
- Enzyme Inhibitors pharmacology MeSH
- Hydrogen-Ion Concentration MeSH
- Molecular Weight MeSH
- Enzyme Stability MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chitin MeSH
- Chitosan MeSH
- chitosanase MeSH Browser
- Glycoside Hydrolases MeSH
- Enzyme Inhibitors MeSH
The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23%) out of 77 crystalliferous strains of Bacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 degrees C with shaking in a medium of pH 6.5 with 4% colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase of B. thuringiensis.
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