Detection of cellulolytic bacteria from the human colon
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15227792
DOI
10.1007/bf02931396
Knihovny.cz E-resources
- MeSH
- Bacteria classification genetics isolation & purification metabolism MeSH
- Bacteroides classification genetics isolation & purification metabolism MeSH
- Bifidobacterium classification genetics isolation & purification metabolism MeSH
- Cellulase biosynthesis MeSH
- Cellulose metabolism MeSH
- Clostridium classification genetics isolation & purification metabolism MeSH
- DNA, Bacterial analysis isolation & purification MeSH
- DNA Fingerprinting MeSH
- Electrophoresis, Polyacrylamide Gel methods MeSH
- Eubacterium classification genetics isolation & purification metabolism MeSH
- Genes, rRNA genetics MeSH
- Colon microbiology MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- DNA, Ribosomal analysis isolation & purification MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Ruminococcus classification genetics isolation & purification metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cellulase MeSH
- Cellulose MeSH
- DNA, Bacterial MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
The main representatives of bacteria in the human colon were investigated by specific PCR and denaturing gradient gel electrophoresis (DGGE). Prevalent in both cases were species of Bifidobacterium, Clostridium, Bacteroides, Faecalibacterium and Eubacterium. Simultaneously, cellulolytic bacteria were isolated from the human feces. The largest proportion was represented by ruminococcus-like isolates. Their presence was confirmed both by PCR and DGGE methods; the latter one was able to give more comprehensive data about the composition of bacterial population in the human colon chyme.
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