Four monoclonal antibodies recognizing TBEV proteins were prepared. Three of them (2/H6, 8/A10, 5/F9) specifically recognize the structural E protein and the fourth binds to the nonstructural NS1 protein. These antibodies were used for an immunofluorescence study of TBEV protein distribution in infected mammalian host and tick vector tissue culture cells. Any differences in the distribution of the E and NS1 proteins were revealed. In porcine PS cell line the proteins were localized in the cytoplasm with a distinct increase of the signal in the perinuclear area 16 h post-infection (p.i.). The area gradually expanded and at 24 h p.i. covered the major part of the cytoplasm. The localization of the proteins in tick RA-257 cells revealed a uniform spread of the proteins in whole cytoplasm of the infected cells. The signal was very weak at 16 h p.i. and gradually increased during progressing time p.i., although, the distribution of the proteins did not exhibit any changes. Difference in viral protein distribution in the two cell types points to the possibility that the maturation process of TBEV exhibits different features in mammalian and tick cells.

Institute of Molecular Genetics Academy of Sciences of the Czech Republic 166 37 Prague Czechia

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J Virol. 1995 Feb;69(2):695-700 PubMed

J Gen Virol. 1989 Feb;70 ( Pt 2):335-43 PubMed

Arch Virol Suppl. 1994;9:339-48 PubMed

Virology. 1968 Mar;34(3):435-43 PubMed

Annu Rev Microbiol. 1990;44:649-88 PubMed

Arch Virol. 1989;106(1-2):103-20 PubMed

Adv Virus Res. 1987;33:45-90 PubMed

Arch Virol. 1987;96(3-4):135-51 PubMed

Nature. 1975 Aug 7;256(5517):495-7 PubMed

Arch Virol. 1995;140(1):145-56 PubMed

J Gen Virol. 1987 Mar;68 ( Pt 3):853-7 PubMed

History of Arbovirus Research in the Czech Republic

Ixodes scapularis and Ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis