Disruption of the RAD51 gene sensitizes S. cerevisiae cells to the toxic and mutagenic effects of hydrogen peroxide
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15259765
DOI
10.1007/bf02931040
Knihovny.cz E-resources
- MeSH
- DNA-Binding Proteins physiology MeSH
- DNA Repair drug effects MeSH
- Hydrogen Peroxide pharmacology MeSH
- DNA Damage drug effects MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Rad51 Recombinase MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Saccharomyces cerevisiae drug effects genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA-Binding Proteins MeSH
- Hydrogen Peroxide MeSH
- RAD51 protein, S cerevisiae MeSH Browser
- Rad51 Recombinase MeSH
- Saccharomyces cerevisiae Proteins MeSH
The RAD51 gene was disrupted in three different parental wild-type strains to yield three rad51 null strains with different genetic background. The rad51 mutation sensitizes yeast cells to the toxic and mutagenic effects of H2O2, suggesting that Rad51-mediated repair, similarly to that of RecA-mediated, is relevant to the repair of oxidative damage in S. cerevisiae. Moreover, pulsed-field gel electrophoresis analysis demonstrated that increased sensitivity of the rad51 mutant to H2O2 is accompanied by its decreased ability to repair double-strand breaks induced by this agent. Our results show that ScRad51 protects yeast cells from H2O2-induced DNA double-strand breakage.
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