Disruption of the RAD51 gene sensitizes S. cerevisiae cells to the toxic and mutagenic effects of hydrogen peroxide
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15259765
DOI
10.1007/bf02931040
Knihovny.cz E-zdroje
- MeSH
- DNA vazebné proteiny fyziologie MeSH
- oprava DNA účinky léků MeSH
- peroxid vodíku farmakologie MeSH
- poškození DNA účinky léků MeSH
- pulzní gelová elektroforéza MeSH
- rekombinasa Rad51 MeSH
- Saccharomyces cerevisiae - proteiny MeSH
- Saccharomyces cerevisiae účinky léků genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA vazebné proteiny MeSH
- peroxid vodíku MeSH
- RAD51 protein, S cerevisiae MeSH Prohlížeč
- rekombinasa Rad51 MeSH
- Saccharomyces cerevisiae - proteiny MeSH
The RAD51 gene was disrupted in three different parental wild-type strains to yield three rad51 null strains with different genetic background. The rad51 mutation sensitizes yeast cells to the toxic and mutagenic effects of H2O2, suggesting that Rad51-mediated repair, similarly to that of RecA-mediated, is relevant to the repair of oxidative damage in S. cerevisiae. Moreover, pulsed-field gel electrophoresis analysis demonstrated that increased sensitivity of the rad51 mutant to H2O2 is accompanied by its decreased ability to repair double-strand breaks induced by this agent. Our results show that ScRad51 protects yeast cells from H2O2-induced DNA double-strand breakage.
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