Geographically and temporally distant natural recombinant isolates of Plum pox virus (PPV) are genetically very similar and form a unique PPV subgroup
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
15302961
DOI
10.1099/vir.0.80206-0
Knihovny.cz E-zdroje
- MeSH
- fylogeneze MeSH
- genom virový * MeSH
- hmyz - vektory MeSH
- listy rostlin MeSH
- molekulární sekvence - údaje MeSH
- mšice MeSH
- nemoci rostlin virologie MeSH
- rekombinace genetická MeSH
- sekvenční homologie MeSH
- slivoň virologie MeSH
- tabák MeSH
- virové geny MeSH
- virové plášťové proteiny genetika MeSH
- virulence genetika MeSH
- virus šarky švestky klasifikace genetika patogenita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Evropa MeSH
- Názvy látek
- virové plášťové proteiny MeSH
Natural recombinant Plum pox virus (PPV) isolates were detected in Albania, Bulgaria, Czech Republic, Germany, Hungary and Slovakia. Despite different geographical origins and dates of isolation, all the recombinant isolates were closely related at the molecular level and shared the same recombination breakpoint as well as a typical signature in their N-terminal coat protein sequence, suggesting a common origin. Biological assays with four recombinant isolates demonstrated their capacity to be aphid-transmitted to various Prunus hosts. One of these isolates had a threonine-to-isoleucine mutation in the conserved PTK motif of its HC-Pro and showed a drastically decreased, although not abolished, aphid transmissibility. The complete genome sequence of one of the recombinant isolates, BOR-3, was determined, as well as some partial sequences in the HC-Pro and P3 genes for additional natural recombinant isolates. Analysis of the phylogenetic relationships between the recombinant isolates and other sequenced PPV isolates confirmed that the recombinant isolates form a phylogenetically homogeneous lineage. In addition, this analysis revealed an ancient recombination event between the PPV-D and M subgroups, with a recombination breakpoint located in the P3 gene. Taken together, these results indicate that recombinant isolates represent an evolutionarily successful, homogeneous group of isolates with a common history and unique founding recombination event. The name PPV-Rec is proposed for this coherent ensemble of isolates.
Citace poskytuje Crossref.org
GENBANK
AJ566344, AJ566345, AJ566346, AJ620686, AJ620687, AY028309, AY324837, AY324838, AY324839, AY324840, AY324841, AY324842, AY324843, AY324844, AY324845, AY324846, AY324847, AY324848, AY553368, AY553369, AY553370, AY553371, AY553372, AY553373, AY553374, AY553375, AY553376, AY553377
