Planktonic and biofilm cells of a clinical urinary isolate of P. aeruginosa were compared in vitro for their ability to adhere to uroepithelial cells, interaction with macrophages, and for production of virulence factors like extracellular proteinase, elastase, hemolysin, phospholipase C and pyochelin. Biofilm cells showed increased adherence to UECs, which was coupled with reduced uptake and intracellular killing by macrophages. Overall there was a decrease in production of extracellular products by biofilm cells. Comparing the two cell forms for their ability to establish infection in an ascending model of acute pyelonephritis, significant enhancement of renal bacterial load, as well as more pronounced renal pathology developed with biofilm cells.

Department of Microbiology Panjab University Post Graduate Institute of Medical Education and Research Chandigarh India

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Infect Immun. 1988 Oct;56(10):2623-30 PubMed

J Bacteriol. 1980 Jun;142(3):836-42 PubMed

J Med Microbiol. 1987 Dec;24(4):363-70 PubMed

J Infect Dis. 1991 Jan;163(1):143-9 PubMed

Folia Microbiol (Praha). 2002;47(4):445-9 PubMed

Infect Immun. 1984 Mar;43(3):1058-63 PubMed

Can J Microbiol. 2001 Dec;47(12):1095-100 PubMed

Science. 1999 Mar 19;283(5409):1837, 1839 PubMed

Anal Biochem. 1972 Nov;50(1):163-73 PubMed

Infect Immun. 1983 Apr;40(1):273-83 PubMed

Infect Immun. 1985 Mar;47(3):723-9 PubMed

Microbiol Immunol. 2001;45(1):9-15 PubMed

Urol Res. 1991;19(5):301-4 PubMed

Chemotherapy. 1997 Mar-Apr;43(2):137-41 PubMed

J Gen Microbiol. 1988 Jan;134(1):29-36 PubMed

Antonie Van Leeuwenhoek. 1986;52(2):153-61 PubMed

Microbes Infect. 2001 Jan;3(1):23-35 PubMed

Indian J Med Res. 2000 Sep;112:93-9 PubMed

Folia Microbiol (Praha). 2003;48(4):529-33 PubMed

Microbiol Rev. 1983 Mar;47(1):46-83 PubMed

Folia Microbiol (Praha). 2003;48(5):671-8 PubMed

MMWR CDC Surveill Summ. 1983;32(4):1SS-16SS PubMed

J Control Release. 1999 Feb 1;57(2):151-9 PubMed

J Antimicrob Chemother. 1984 May;13 Suppl B:85-8 PubMed

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