Viability, ultrastructure and cytokinin metabolism of free and immobilized tobacco chloroplasts
Language English Country Netherlands Media print
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15604795
DOI
10.1023/b:bile.0000045651.56662.fc
PII: DO00002640
Knihovny.cz E-resources
- MeSH
- Cell Culture Techniques methods MeSH
- Chloroplasts physiology ultrastructure MeSH
- Cytokinins metabolism MeSH
- Cells, Immobilized physiology ultrastructure MeSH
- Cells, Cultured MeSH
- Nicotiana physiology ultrastructure MeSH
- Cell Survival physiology MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Cytokinins MeSH
Cytokinins play a decisive role in regulation of plastid development and differentiation, but their metabolism in plastids is not known. Metabolic studies using intact chloroplasts are prevented by their instability once they are isolated from leaf cells. Chloroplasts of Nicotiana tabacum L. cv. Petit Havana SR1 were therefore immobilized into low-viscosity alginate. Their intactness was assessed by a glyceraldehyde-3-phosphate dehydrogenase assay which indicated that free chloroplasts totally disintegrated within 7 h, while more than 50% of immobilized chloroplasts remained intact after 24 h. The immobilization had no marked impact on ultrastructure and postponed final destruction. The metabolite profile was similar in free and immobilized chloroplasts after 4 h incubation with tritiated zeatin. Nevertheless, the yield of conversion products decreased twice in immobilized chloroplasts, which was probably the outcome of mass transfer limitations and/or the sorption to polysaccharide matrix.
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