Viability, ultrastructure and cytokinin metabolism of free and immobilized tobacco chloroplasts
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
15604795
DOI
10.1023/b:bile.0000045651.56662.fc
PII: DO00002640
Knihovny.cz E-zdroje
- MeSH
- buněčné kultury metody MeSH
- chloroplasty fyziologie ultrastruktura MeSH
- cytokininy metabolismus MeSH
- imobilizované buňky fyziologie ultrastruktura MeSH
- kultivované buňky MeSH
- tabák fyziologie ultrastruktura MeSH
- viabilita buněk fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- cytokininy MeSH
Cytokinins play a decisive role in regulation of plastid development and differentiation, but their metabolism in plastids is not known. Metabolic studies using intact chloroplasts are prevented by their instability once they are isolated from leaf cells. Chloroplasts of Nicotiana tabacum L. cv. Petit Havana SR1 were therefore immobilized into low-viscosity alginate. Their intactness was assessed by a glyceraldehyde-3-phosphate dehydrogenase assay which indicated that free chloroplasts totally disintegrated within 7 h, while more than 50% of immobilized chloroplasts remained intact after 24 h. The immobilization had no marked impact on ultrastructure and postponed final destruction. The metabolite profile was similar in free and immobilized chloroplasts after 4 h incubation with tritiated zeatin. Nevertheless, the yield of conversion products decreased twice in immobilized chloroplasts, which was probably the outcome of mass transfer limitations and/or the sorption to polysaccharide matrix.
Citace poskytuje Crossref.org
