Retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor
Language English Country Czech Republic Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15641927
PII: 705
Knihovny.cz E-resources
- MeSH
- Cell Differentiation drug effects physiology MeSH
- Embryo, Mammalian MeSH
- Interleukin-6 pharmacology MeSH
- Leukemia Inhibitory Factor MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Neurons cytology drug effects MeSH
- Drug Synergism MeSH
- Tretinoin pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Interleukin-6 MeSH
- Leukemia Inhibitory Factor MeSH
- Lif protein, mouse MeSH Browser
- Tretinoin MeSH
Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferation, survival and differentiation in a wide range of cell types. Here we show that LIF potentiates retinoic acid-mediated neural induction in pluripotent P19 embryonal carcinoma cells. This activity of LIF was demonstrated by a profounded neural morphology followed by increased expression of neural-specific proteins (N-CAM, III beta-tubulin, and GAP-43), up-regulation of early neural lineage-specific gene Mash-1, and down-regulation of early endoderm-specific genes -fetoprotein and GATA-4. Moreover, LIF also slows growth and increases the level of apoptosis in differentiating cells.
