Rapid G+ count and subpopulation assessment of the intestinal bacteria in Apodemus sylvaticus and Mus musculus by flow cytometry
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15702550
DOI
10.1007/bf02931538
Knihovny.cz E-resources
- MeSH
- Bacteria classification isolation & purification MeSH
- Fluorescent Dyes MeSH
- Muridae microbiology MeSH
- Mice microbiology MeSH
- Organic Chemicals MeSH
- Colony Count, Microbial methods MeSH
- Flow Cytometry methods MeSH
- Intestines microbiology MeSH
- Bacterial Typing Techniques MeSH
- Animals MeSH
- Check Tag
- Mice microbiology MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fluorescent Dyes MeSH
- Organic Chemicals MeSH
- SYTO 13 MeSH Browser
We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial population and to describe bacterial subpopulations in the intestine.
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