Immunochemical analysis of lipopolysaccharide-like component extracted from Borrelia burgdorferi sensu lato
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
15702557
DOI
10.1007/bf02931545
Knihovny.cz E-zdroje
- MeSH
- Borrelia burgdorferi komplex chemie genetika imunologie MeSH
- Borrelia burgdorferi chemie imunologie MeSH
- Borrelia chemie imunologie MeSH
- DNA bakterií genetika MeSH
- druhová specificita MeSH
- imunoelektroforéza dvourozměrná MeSH
- imunoelektroforéza MeSH
- králíci MeSH
- lipopolysacharidy analýza imunologie MeSH
- protilátky bakteriální MeSH
- sekvence nukleotidů MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- DNA bakterií MeSH
- lipopolysacharidy MeSH
- protilátky bakteriální MeSH
Immunoelectrophoresis and its modifications were applied to analysis of a lipopolysaccharide-like component (LPS-LC) extracted from Borrelia garinii strains K24 and K48 isolated from Ixodes ricinus and Borrelia burgdorferi sensu stricto strain B31. A modification of the hot phenol-water method was used for isolation of LPS. Immunoelectrophoresis (IE) and crossed immunoelectrophoresis (CIE) of LPS-LC with polyclonal rabbit antisera revealed a pattern and properties partially similar to LPS from other Gram-negative bacteria. B. garinii LPS-LC formed in CIE a diffuse band extending from the start to the anode. Similarly, the shape and position of the band in IE did not show major differences from LPS of other Gram-negative bacteria. The LPS-LC extracted from the three genomic groups of B. burgdorferi sensu lato were found to have similar immunochemical properties irrespective of their genotype origin.
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