Cryptic species within the Tetratrichomonas gallinarum species complex revealed by molecular polymorphism
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15725528
DOI
10.1016/j.vetpar.2004.11.003
PII: S0304-4017(04)00495-9
Knihovny.cz E-resources
- MeSH
- Anseriformes * MeSH
- Phylogeny MeSH
- Galliformes * MeSH
- Humans MeSH
- DNA, Ribosomal Spacer chemistry genetics MeSH
- Molecular Sequence Data MeSH
- Bird Diseases parasitology MeSH
- Polymerase Chain Reaction veterinary MeSH
- DNA, Protozoan chemistry genetics MeSH
- RNA, Ribosomal, 16S chemistry genetics MeSH
- RNA, Ribosomal, 5.8S chemistry genetics MeSH
- Base Sequence MeSH
- Sequence Alignment MeSH
- Cluster Analysis MeSH
- Random Amplified Polymorphic DNA Technique veterinary MeSH
- Trichomonas classification genetics growth & development isolation & purification MeSH
- Trichomonas Infections parasitology veterinary MeSH
- Zoonoses parasitology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Ribosomal Spacer MeSH
- DNA, Protozoan MeSH
- RNA, Ribosomal, 16S MeSH
- RNA, Ribosomal, 5.8S MeSH
Tetratrichomonas gallinarum is a widespread intestinal parasite of galliform and anseriform birds. The pathogenicity of this species is controversial, presenting an unsettled problem as yet. We analysed the polymorphism and genetic relationship among 29 isolates of T. gallinarum obtained from eight bird species and five T. gallinarum-like isolates from the oral cavity and lower respiratory tract of human patients. Two methods were used for the analyses: RAPD and sequencing of 16S rRNA, 5.8S rRNA, ITS1 and ITS2 genes, both producing consistent and well-supported results. The isolates were divided into five groups, A-E, with eleven subgroups. The distance between groups E, D and the cluster A-B-C considerably exceeded usual intraspecific polymorphism seen in trichomonads. Moreover, the largest subgroup, A2 (containing 18 isolates), was divided into three branches according to the host specificity. All isolates from humans were placed into avian subgroups A2 and B2. We conclude that our isolates represent, at least, three morphospecies or rather complexes of several cryptic species. Since certain species of the T. gallinarum complex can differ in their biological characteristics and some of them can infect humans, the problem of T. gallinarum pathogenicity should be re-examined with regard to specific genetic groups and zoonotic potential of some of these lineages should be considered.
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