Cryptic species within the Tetratrichomonas gallinarum species complex revealed by molecular polymorphism
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15725528
DOI
10.1016/j.vetpar.2004.11.003
PII: S0304-4017(04)00495-9
Knihovny.cz E-zdroje
- MeSH
- Anseriformes * MeSH
- fylogeneze MeSH
- Galliformes * MeSH
- lidé MeSH
- mezerníky ribozomální DNA chemie genetika MeSH
- molekulární sekvence - údaje MeSH
- nemoci ptáků parazitologie MeSH
- polymerázová řetězová reakce veterinární MeSH
- protozoální DNA chemie genetika MeSH
- RNA ribozomální 16S chemie genetika MeSH
- RNA ribozomální 5.8S chemie genetika MeSH
- sekvence nukleotidů MeSH
- sekvenční seřazení MeSH
- shluková analýza MeSH
- technika náhodné amplifikace polymorfní DNA veterinární MeSH
- Trichomonas klasifikace genetika růst a vývoj izolace a purifikace MeSH
- trichomoniáza parazitologie veterinární MeSH
- zoonózy parazitologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- mezerníky ribozomální DNA MeSH
- protozoální DNA MeSH
- RNA ribozomální 16S MeSH
- RNA ribozomální 5.8S MeSH
Tetratrichomonas gallinarum is a widespread intestinal parasite of galliform and anseriform birds. The pathogenicity of this species is controversial, presenting an unsettled problem as yet. We analysed the polymorphism and genetic relationship among 29 isolates of T. gallinarum obtained from eight bird species and five T. gallinarum-like isolates from the oral cavity and lower respiratory tract of human patients. Two methods were used for the analyses: RAPD and sequencing of 16S rRNA, 5.8S rRNA, ITS1 and ITS2 genes, both producing consistent and well-supported results. The isolates were divided into five groups, A-E, with eleven subgroups. The distance between groups E, D and the cluster A-B-C considerably exceeded usual intraspecific polymorphism seen in trichomonads. Moreover, the largest subgroup, A2 (containing 18 isolates), was divided into three branches according to the host specificity. All isolates from humans were placed into avian subgroups A2 and B2. We conclude that our isolates represent, at least, three morphospecies or rather complexes of several cryptic species. Since certain species of the T. gallinarum complex can differ in their biological characteristics and some of them can infect humans, the problem of T. gallinarum pathogenicity should be re-examined with regard to specific genetic groups and zoonotic potential of some of these lineages should be considered.
Citace poskytuje Crossref.org
GENBANK
AY245106, AY245107, AY245108, AY245109, AY245110, AY245111, AY245112, AY245113, AY245114, AY245115, AY245116, AY245117, AY245118, AY245119, AY245120, AY245121, AY245122, AY245123, AY245124, AY245125, AY245126, AY245127, AY245128, AY245129, AY245130, AY245131, AY245132, AY245133, AY245134, AY245135, AY245136, AY245137, AY245138, AY245139, AY245140, AY245141, AY245142, AY245143, AY245144, AY245145, AY245146, AY245147, AY245148, AY245149, AY245150, AY245151, AY245152, AY245153, AY245154, AY245155, AY245156, AY245157, AY245158, AY245159, AY245160, AY245161, AY245162, AY245163
