Trichomonads are known to inhabit the oral cavities of various mammals, including dogs, cats and horses. However, little attention has been paid to species identification, prevalence and zoonotic potential of these parasites, although their hosts live in close proximity with humans. According to the original description, oral trichomonads in dogs and cats belong to the genus Tetratrichomonas. Interestingly, later investigations suggested that the oral cavities of dogs and cats could be infected with different species of the genus Trichomonas, including the human oral cavity parasite Trichomonas tenax. Thus, in this study we investigated the occurrence of oral trichomonads in 111 domestic dogs and 122 cats using cell cultivation methods, nested PCR analyses, and the sequencing of ITS1-5.8rRNA-ITS2 regions. We found that both dogs and cats harbour T. tenax, with prevalences of 8.1% and 4.1%, respectively. Considerably more dogs were infected with different species of the genus Trichomonas (30.6%), which we also identified in cats (6.6%). An analysis of the potential risk factors suggested that dogs of more than 3years old or with dental disease signs are more frequently infected with Trichomonas sp. than younger dogs or dogs without the disease signs, and that crossbreed dogs revealed increased rates of infection in comparison with purebred dogs. An analysis of the cat population suggested that Trichomonas sp. infection is lower in younger and crossbreed cats. Although the morphology of Trichomonas sp. is very similar to that of T. tenax, based on a phylogenetic analysis of ITS1-5.8rRNA-ITS2 regions and the ssrRNA genes, we consider Trichomonas sp. to represent a new trichomonad species, for which we propose the name Trichomonas brixi.
- MeSH
- fylogeneze MeSH
- kočky MeSH
- koně MeSH
- lidé MeSH
- nemoci koček epidemiologie parazitologie MeSH
- nemoci koní parazitologie MeSH
- nemoci psů epidemiologie parazitologie MeSH
- prevalence MeSH
- protozoální DNA genetika MeSH
- psi MeSH
- RNA protozoální genetika MeSH
- sekvence nukleotidů MeSH
- Trichomonas klasifikace cytologie izolace a purifikace MeSH
- trichomoniáza epidemiologie parazitologie veterinární MeSH
- ústa parazitologie MeSH
- zoonózy epidemiologie parazitologie MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- lidé MeSH
- mužské pohlaví MeSH
- psi MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
The efficacy of the OSOM Trichomonas Rapid Test (developed for rapid diagnosis of human Trichomonas vaginalis) in detection of Trichomonas spp. in pigeons (Columba livia) was investigated. Two oral cavity swabs were taken from 50 farm pigeons. Cultivation in Diamond Trichomonas medium was used as a reference method. According to a morphological determination, Trichomonas gallinae was the only protozoan found; however, no further molecular analysis was conducted. The OSOM Trichomonas test was positive in 39 oral swabs. In comparison with the cultivation method three samples were identified as false negative and one as false positive. Test specificity and sensitivity were established as 93% and 90%, respectively. Using Cohen's Kappa, the concordance between the two testing methods was found to be strong (kappa = 0.7506, 95% CI = 0.5162-0.9850). The OSOM Trichomonas test is not able to distinguish between Trichomonas species; however, results suggest that the test is suitable for the rapid detection of Trichomonas spp. infection in pigeons.
- MeSH
- chromatografie afinitní metody veterinární MeSH
- Columbidae * MeSH
- nemoci ptáků diagnóza parazitologie MeSH
- Trichomonas izolace a purifikace MeSH
- trichomoniáza diagnóza parazitologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- skot MeSH
- trichomoniáza veterinární MeSH
- Check Tag
- skot MeSH
Nedostatky terapie etiotropní metodou. Nová komplexní metoda a její nejdůležitější zásady. (Zlepšení celkového zdravotního stavu dobytčete, prostředky k odstranění patologického obsahu dělohy a prostředky k regeneraci fu|nkce pohlav. aparátu). Dosažené výsledky
- MeSH
- nemoci skotu terapie MeSH
- skot MeSH
- trichomoniáza terapie veterinární MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
