A novel HLA-B*420502 allele identified by PCR-SSO/SSP routine typing and confirmed by Sequencing-based typing
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15730521
DOI
10.1111/j.1399-0039.2005.00376.x
PII: TAN376
Knihovny.cz E-resources
- MeSH
- Alleles * MeSH
- White People genetics MeSH
- Point Mutation MeSH
- HLA-B Antigens genetics MeSH
- Leukemia genetics MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Histocompatibility Testing MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- HLA-B Antigens MeSH
- HLA-B*42:05:02 antigen MeSH Browser
A novel human leukocyte antigen-B (HLA-B) allele, B*420502, was identified in a patient with leukemia (Caucasoid, Czech ancestry) and his mother during intrafamily search for the hematopoietic stem cell donor. The novel allele was initially detected by HLA typing at low resolution using both sequence specific primers and sequence specific oligonucleotides techniques that resulted in unique reaction patterns. The alleles of the HLA-B locus were separated by the haplotype-specific extraction technique. Sequencing of those alleles revealed a novel allele, B*420502, that is identical with B*420501 except a T-->G exchange (synonymous mutation) at position 618.
References provided by Crossref.org
GENBANK
AJ716156, AJ716157, AJ716158
