A flavivirus (strain 97-103) was isolated from Culex pipens mosquitoes in 1997 following floods in South Moravia, Czech Republic. The strain exhibited close antigenic relationship to West Nile virus (WNV) prototype strain Eg-101 in a cross-neutralization test. In this study, mouse pathogenicity characteristics and the complete nucleotide and putative amino acid sequences of isolate 97-103, named Rabensburg virus (RabV) after a nearby Austrian city, were determined. RabV shares only 75%-77% nucleotide identity and 89%-90% amino acid identity with representative strains of WNV lineages 1 and 2. Another RabV strain (99-222) was isolated in the same location 2 years later; it showed >99% nucleotide identity to strain 97-103. Phylogenetic analyses of RabV, WNV strains, and other members of the Japanese encephalitis virus (JEV) complex clearly demonstrated that RabV is either a new (third) lineage of WNV or a novel flavivirus of the JEV group.

University of Veterinary Medicine A 1210 Vienna Austria

Zobrazit více v PubMed

Heinz FX, Collett MS, Purcell RH, Gould EA, Howard CR, Houghton M, et al. Family Flaviviridae. In: van Regenmortel MHV, Faquet CM, Bishop DHL, editors. Virus taxonomy, Seventh International Committee for the Taxonomy of Viruses. San Diego: Academic Press; 2000. p. 859–78.

Hayes CG. West Nile fever. In: Monath TP, editor. The arboviruses: epidemiology and ecology. Vol. V. Boca Raton (FL): CRC Press; 1989. p. 59–88.

Hubálek Z, Halouzka J. West Nile fever—a reemerging mosquito-borne viral disease in Europe. Emerg Infect Dis. 1999;5:643–50. 10.3201/eid0505.990506 PubMed DOI PMC

Hubálek Z. European experience with the West Nile virus ecology and epidemiology: could it be relevant for the New World? Viral Immunol. 2000;13:415–26. 10.1089/vim.2000.13.415 PubMed DOI

Murgue B, Zeller H, Deubel V. The ecology and epidemiology of West Nile virus in Africa, Europe. In: Mackenzie JS, Barrett ADT, Deubel V, editors. Japanese encephalitis and West Nile viruses. Current topics in Microbiology. Vol. 267: West Nile. Berlin: Springer; 2002. p. 195–221. PubMed

Zeller HG, Schuffnecker I. West Nile virus: an overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur J Clin Microbiol Infect Dis. 2004;23:147–56. 10.1007/s10096-003-1085-1 PubMed DOI

Berthet F, Zeller HG, Drouet M, Rauzier J, Digoutte J, Deubel V. Extensive nucleotide changes and deletions within the envelope gene of Euro-African West Nile viruses. J Gen Virol. 1997;78:2293–7. PubMed

Savage HM, Ceianu C, Nicolescu G, Karabatsos N, Lanciotti RS, Vladimirescu A, et al. Entomologic and avian investigations of an epidemic of West Nile fever in Romania, 1996, with serologic and molecular characterization of a virus from mosquitoes. Am J Trop Med Hyg. 1999;61:600–11. PubMed

Lanciotti RS, Roehrig JT, Deubel V, Smith J, Parker M, Steele K, et al. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern U.S. Science. 1999;286:2333–7. PubMed

Charrel RN, Brault AC, Gallian P, Lemasson JJ, Murgue B, Murri S, et al. Evolutionary relationship between Old World West Nile virus strains evidence for viral gene flow between Africa, the Middle East, and Europe. Virology. 2003;315:381–8. 10.1016/S0042-6822(03)00536-1 PubMed DOI

Hubálek Z, Halouzka J, Juricova Z. West Nile fever in Czechland. Emerg Infect Dis. 1999;5:594–5. 10.3201/eid0504.990430 PubMed DOI PMC

Ferenzi E, Bakonyi T, Tóth-Mittler E, Czeglédi A, Bán E. Emergence of an old-new virus: domestic West Nile virus infections with central nervous system symptoms, 2003–2004. In: Abstracts of the 2004 year Congress of the Hungarian Society for Microbiology; 2004. Oct. 7–9, Keszthely, Hungary. Abstract p. 36–7 [in Hungarian]. Budapest: Hungarian Soceity for Microbiology; 2004.

Hubálek Z, Halouzka J, Juricova Z, Sebesta O. First isolation of mosquito-borne West Nile virus in the Czech Republic. Acta Virol. 1998;42:119–20. PubMed

Hubálek Z, Savage HM, Halouzka J, Juricova Z, Sanogo YO, Lusk S. West Nile virus investigations in South Moravia, Czechland. Viral Immunol. 2000;13:427–33. 10.1089/vim.2000.13.427 PubMed DOI

Bakonyi T, Gould EA, Kolodziejek J, Weissenböck H, Nowotny N. Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001; comparing with the South African strain SAAR-1776 and other flaviviruses. Virology. 2004;328:301–10. 10.1016/S0042-6822(04)00525-2 PubMed DOI

Melnick JL, Paul JR, Riordan JT, Barnett VH, Goldblum N, Zabin E. Isolation from human sera in Egypt of a virus apparently identical to West Nile virus. Proc Soc Exp Biol Med. 1951;77:661–5. PubMed

Rice CM, Lenches EM, Eddy SR, Shin SJ, Sheets RL, Strauss JH. Nucleotide sequence of yellow fever virus: Implications for flavivirus gene expression and evolution. Science. 1985;229:726–33. 10.1126/science.4023707 PubMed DOI

Lanciotti RS, Ebel GD, Deubel V, Kerst AJ, Murri S, Meyer R, et al. Complete genome sequences and phylogenetic analysis of West Nile virus strains isolated from the United States, Europe, and the Middle East. Virology. 2002;298:96–105. 10.1006/viro.2002.1449 PubMed DOI

Prilipov AG, Kinney RM, Samokhvalov EI, Savage HM, Al'khovskii SV, Tsuchiya KR, et al. Analysis of new variants of West Nile fever virus [in Russian]. Vopr Virusol. 2002;47:36–41. PubMed

Lvov DK, Kovtunov AI, Iashkulov KB, Gromashevskii VL, Dzharkenov AF, Shchelkanov MI, et al. Circulation of West Nile virus (Flaviviridae, Flavivirus) and some other arboviruses in the ecosystems of Volga delta, Volga-Akhtuba flood-lands and adjoining arid regions (2000–2002) [in Russian]. Vopr Virusol. 2004;49:45–51. PubMed

Lvov DK, Butenko AM, Gromashevsky VL, Kovtunov AI, Prilipov AG, Kinney R, et al. West Nile virus and other zoonotic viruses in Russia: examples of emerging-reemerging situations. Arch Virol Suppl. 2004;18:85–96. PubMed

Kuno G, Chang GJ, Tsuchiya KR, Karabatsos N, Cropp CB. Phylogeny of the genus Flavivirus. J Virol. 1998;72:73–83. PubMed PMC

Burke DS, Monath TP. Flaviviruses. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, et al. editors. Fields virology, vol. 1. Philadelphia: Lippincott Williams & Wilkins; 2001. p. 1055–109.

Gaunt MW, Sall AA, de Lamballerie X, Falconar AK, Dzhivanian TI, Gould EA. Phylogenetic relationships of flaviviruses correlate with their epidemiology, disease association and biogeography. J Gen Virol. 2001;82:1867–76. PubMed

History of Arbovirus Research in the Czech Republic

West Nile virus in overwintering mosquitoes, central Europe