Characteristic of two mouse bcr-abl-transformed cell lines: I. General properties of the cells
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15783087
PII: file/6206/fb2005a0003.pdf
Knihovny.cz E-resources
- MeSH
- Fusion Proteins, bcr-abl genetics metabolism MeSH
- Benzamides MeSH
- Drug Resistance, Neoplasm physiology MeSH
- Down-Regulation physiology MeSH
- Imatinib Mesylate MeSH
- Neoplasm Invasiveness physiopathology MeSH
- Leukemia drug therapy metabolism pathology MeSH
- Histocompatibility Antigens Class I metabolism MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Cell Transformation, Neoplastic metabolism MeSH
- Biomarkers, Tumor metabolism MeSH
- Piperazines pharmacology MeSH
- Cell Proliferation drug effects MeSH
- Antineoplastic Agents pharmacology MeSH
- Pyrimidines pharmacology MeSH
- Cellular Senescence physiology MeSH
- Cell Line, Transformed metabolism pathology transplantation MeSH
- Neoplasm Transplantation MeSH
- Cell Shape MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Fusion Proteins, bcr-abl MeSH
- Benzamides MeSH
- Imatinib Mesylate MeSH
- Histocompatibility Antigens Class I MeSH
- Biomarkers, Tumor MeSH
- Piperazines MeSH
- Antineoplastic Agents MeSH
- Pyrimidines MeSH
In an effort to develop an experimental system suitable for immunological studies in which Bcr-Abl-positive cells are to be used as antigens, we examined the properties of two mouse (Balb/c) established cell lines that express the Bcr-Abl protein and are oncogenic for syngeneic animals. Under standard conditions the two cell lines, viz. Ba-p210 (B210) and 12B1, expressed comparable amounts of the Bcr-Abl protein. However, they differed in a number of characteristics. From the morphological point of view, B210 cells were the more homogeneous, being mainly represented by leukaemic blastic cells with a large number of AgNORs as markers indicating a high proliferative activity. 12B1 cells were more polymorphic and giant cells were detected within their populations. Many 12B1 cells exhibited nuclear segmentation and "band-like" structures. Markers of proliferation were less frequent in 12B1 and the tendency for aging was more pronounced in these cells. The 12B1 cells were slightly more sensitive to imatinib mesylate than B210 cells. In B210 cells, the expression of MHC class I was downregulated, which was not the case with 12B1 cells. Both cell lines induced leukaemia-like disease in mice after intravenous application but, as compared with B210, 12B1 cells were about 100 times more oncogenic and the disease they induced was more aggressive. Moreover, 12B1, but not B210, induced tumours after subcutaneous or intraperitoneal inoculation.
Folia Biol (Praha). 2005;51(5):158 PubMed