Coding region single nucleotide polymorphism in the barley low-pI, alpha-amylase gene Amy32b
Language English Country Germany Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- alpha-Amylases genetics MeSH
- DNA Primers MeSH
- Genetic Variation * MeSH
- Genotype MeSH
- Hordeum genetics MeSH
- Polymorphism, Single Nucleotide genetics MeSH
- Chromosome Mapping * MeSH
- Molecular Sequence Data MeSH
- Genes, Plant genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Alignment MeSH
- Amino Acid Substitution genetics MeSH
- Codon, Terminator genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- alpha-Amylases MeSH
- DNA Primers MeSH
- Codon, Terminator MeSH
Barley alpha-amylase variability influences the quality of barley grain in the brewing, feed and food industries. alpha-Amylase proteins are encoded by multigene families in cereals, and this study focused on the barley Amy32b gene. We identified coding region single nucleotide polymorphism (cSNP) and insertion/deletion variation in DNA sequences, which resulted in amino acid substitution and stop codon formation, respectively. The substitution affected the beta1 strand in domain C, whereas the stop codon removed the beta5 strand. Possible effects of these changes on the protein are discussed. A cSNP in the coding region of the Amy32b gene was used as a specific marker to map Amy32b loci on chromosome 7H.
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