Characterization of esculin-positive Pseudomonas fluorescens strains isolated from an underground brook
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15881410
DOI
10.1007/bf02931556
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial analysis MeSH
- Esculin metabolism MeSH
- Fatty Acids analysis MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Pseudomonas fluorescens chemistry classification genetics metabolism MeSH
- DNA Restriction Enzymes metabolism MeSH
- Ribotyping MeSH
- DNA, Ribosomal analysis MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Fresh Water microbiology MeSH
- Bacterial Typing Techniques * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Esculin MeSH
- Fatty Acids MeSH
- DNA Restriction Enzymes MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole-cell fatty-acid methyl-esters analysis (FAME). All strains were phenotypically close to Pseudomonas fluorescens, but they revealed high biochemical variability as well as some reactions atypical for P. fluorescens species. Because identification of pseudomonads by of biochemical testing is often unclear, further techniques were employed. Fingerprints obtained by MERFLP clearly showed that all strains represent P. fluorescens species. Ribotyping separated the strains analyzed into four groups corresponding almost completely (with the exception of one strain) to the clustering based on biochemical profiles. FAME analysis grouped all the strains into one cluster together with the P. putida (biotype A, B), P. chlororaphis and P. fluorescens biotype F representatives, but differentiated them from other FAME profiles of all pseudomonads included in the standard library TSBA 40 provided by MIDI, Inc.
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