Evaluation of ribotyping for characterization and identification of Enterococcus haemoperoxidus and Enterococcus moraviensis strains
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
11557135
DOI
10.1111/j.1574-6968.2001.tb10815.x
PII: S0378109701003342
Knihovny.cz E-zdroje
- MeSH
- deoxyribonukleasa EcoRI genetika MeSH
- deoxyribonukleasa HindIII genetika MeSH
- DNA bakterií analýza MeSH
- druhová specificita MeSH
- Enterococcus klasifikace genetika MeSH
- ribotypizace * MeSH
- sekvenční homologie nukleových kyselin MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- deoxyribonukleasa EcoRI MeSH
- deoxyribonukleasa HindIII MeSH
- DNA bakterií MeSH
Seven Enterococcus moraviensis and 16 Enterococcus haemoperoxidus as well as nine reference cultures of other enterococcal species obtained from the Czech Collection of Microorganisms were characterized using ribotyping with EcoRI and HindIII in the present work. The ribopatterns obtained by both restriction enzymes clearly distinguished all E. moraviensis and E. haemoperoxidus strains from the other enterococci (E. faecalis, E. faecium, E. avium, E. raffinosus, E. pseudoavium, E. malodoratus) and they differentiated both species from each other as well. Although all strains were isolated from different sampling sites, many strains shared the same band patterns. E. moraviensis formed four ribogroups using EcoRI and two ribogroups using HindIII restriction enzyme. E. haemoperoxidus gave six different patterns with EcoRI and five using the HindIII restriction enzyme.
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