Characterization of esculin-positive Pseudomonas fluorescens strains isolated from an underground brook
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15881410
DOI
10.1007/bf02931556
Knihovny.cz E-zdroje
- MeSH
- DNA bakterií analýza MeSH
- eskulin metabolismus MeSH
- mastné kyseliny analýza MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- Pseudomonas fluorescens chemie klasifikace genetika metabolismus MeSH
- restrikční enzymy metabolismus MeSH
- ribotypizace MeSH
- ribozomální DNA analýza MeSH
- RNA ribozomální 16S genetika MeSH
- sladká voda mikrobiologie MeSH
- techniky typizace bakterií * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA bakterií MeSH
- eskulin MeSH
- mastné kyseliny MeSH
- restrikční enzymy MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole-cell fatty-acid methyl-esters analysis (FAME). All strains were phenotypically close to Pseudomonas fluorescens, but they revealed high biochemical variability as well as some reactions atypical for P. fluorescens species. Because identification of pseudomonads by of biochemical testing is often unclear, further techniques were employed. Fingerprints obtained by MERFLP clearly showed that all strains represent P. fluorescens species. Ribotyping separated the strains analyzed into four groups corresponding almost completely (with the exception of one strain) to the clustering based on biochemical profiles. FAME analysis grouped all the strains into one cluster together with the P. putida (biotype A, B), P. chlororaphis and P. fluorescens biotype F representatives, but differentiated them from other FAME profiles of all pseudomonads included in the standard library TSBA 40 provided by MIDI, Inc.
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