PCR-based methods for identification of Enterococcus species
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12630313
DOI
10.1007/bf02818665
Knihovny.cz E-resources
- MeSH
- Species Specificity MeSH
- Enterococcus classification genetics metabolism MeSH
- DNA, Intergenic chemistry genetics MeSH
- Polymerase Chain Reaction methods MeSH
- DNA, Protozoan chemistry genetics MeSH
- Deoxyribonucleases, Type II Site-Specific metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CAGCTG-specific type II deoxyribonucleases MeSH Browser
- DNA, Intergenic MeSH
- DNA, Protozoan MeSH
- Deoxyribonucleases, Type II Site-Specific MeSH
Two DNA-based techniques were used for species identification of enterococci. PvuII digestion of the genus-specific PCR product yielded four different restriction profiles among 20 enterococcal species; one of them was species-specific for E. faecium. In the second case, 32 reference strains belonging to 20 enterococcal species were divided to 12 groups by amplification of internal transcribed spacer of rRNA operon. Interspecies and some intraspecies profile variability was determined. Both methods gave similar results.
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