Genotyping of fimbrial adhesins in Escherichia coli strains isolated from Slovak piglets suffering from diarrhea
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15114867
PubMed Central
PMC7090526
DOI
10.1007/bf02931647
Knihovny.cz E-zdroje
- MeSH
- adheziny Escherichia coli genetika MeSH
- antigeny bakteriální genetika MeSH
- bakteriální adheziny genetika MeSH
- bakteriální toxiny biosyntéza MeSH
- DNA bakterií analýza genetika MeSH
- Escherichia coli genetika izolace a purifikace MeSH
- genotyp MeSH
- hemolyziny biosyntéza MeSH
- infekce vyvolané Escherichia coli mikrobiologie veterinární MeSH
- nemoci prasat mikrobiologie MeSH
- polymerázová řetězová reakce MeSH
- prasata mikrobiologie MeSH
- proteiny z Escherichia coli genetika MeSH
- průjem mikrobiologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Slovenská republika MeSH
- Názvy látek
- adheziny Escherichia coli MeSH
- antigeny bakteriální MeSH
- bakteriální adheziny MeSH
- bakteriální toxiny MeSH
- DNA bakterií MeSH
- F41 antigen, E coli MeSH Prohlížeč
- hemolyziny MeSH
- proteiny z Escherichia coli MeSH
One-hundred sixty Escherichia coli isolates obtained from piglets with diarrhea from different parts of Slovakia were examined for the presence of genes coding for F4, F5, F6 and F41 fimbrial adhesins, and hemolytic activity. According to polymerase chain reaction tests 74 (46%) E. coli isolates were positive for primers that detected genes coding for fimbrial adhesins. Of these 74 isolates, 64 were positive for genes encoding for F4+, four for F5+, five for F6+, and one for both F41+ and F5+ adhesins.
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Alexa P., Štouračová K., Hamřík J., Rychlík I. Gene typing of the colonization factors K88 (F4) in enterotoxigenicEscherichia coli strains isolated from diarrheic piglets. Vet.Med.Czech. 2001;46:46–49.
Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Current Protocols in Molecular Biology. New York: Greene Publishing Association-Wiley-Interscience; 1989.
Balows A., Hausler W.J., Herrmann K.L., Isenberg H.D., Shadomy H.J. Manual of Clinical Microbiology. 5th ed. Washington (DC): American Society for Microbiology; 1991.
Bijlsma I.G.W., Van Houten M., Frik J.F., Ruitenberg E.J. K88 variants K88ab, K88ac and K88id in oral vaccination of different porcine adhesive phenotypes. Immunological aspects. Vet.Immunol.Immunopathol. 1987;16:235–250. doi: 10.1016/0165-2427(87)90020-1. PubMed DOI
Bogyiová E., Kmeťová M., Biroš E., Siegfried L. Detection ofpap-, sfa- andafa-specific DNA sequences inEscherichia coli strains isolated from extraintestinal material. Folia Microbiol. 2002;47:723–726. doi: 10.1007/BF02818678. PubMed DOI
Cryan B. Comparison of the assay systems for detection of enterotoxigenicEscherichia coli heat-stable enterotoxin. J.Clin.Microbiol. 1990;28:792–794. PubMed PMC
Donnenberg M.S. EnteropathogenicEscherichia coli. In: Blaser M.J., Smith P.D., Ravdin J.I., Greenberg H.B., Guerrant R.L., editors. Infection of the Gastrointestinal Tract. New York: Raven Press; 1995. pp. 709–726.
Drahovská H., Kocíncová D., Seman M., Turňa J. PCR-based methods for identification ofEnterococcus species. Folia Microbiol. 2002;47:649–653. doi: 10.1007/BF02818665. PubMed DOI
Dykes C.W., Halliday I.J., Read M.J., Hobden A.N., Harford S. Nucleotide sequences of four variants of the K88 gene of poreine enterotoxigenicEscherichia coli. Infect.Immun. 1985;50:279–283. PubMed PMC
Evans D.G., Evans D.J., Pierce E.F. Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins ofEscherichia coli. Infect.Immun. 1973;7:873–880. PubMed PMC
Frydendahl K. Prevalence of serogroups and virulence genes inEscherichia coli associated with postweaning diarrhea and edema disease in pigs and a comparison of diagnosis approaches. Vet.Microbiol. 2002;85:169–182. doi: 10.1016/S0378-1135(01)00504-1. PubMed DOI
Garabal J.L., Vazquez F., Blanco J., Blanco M., González E.A. Colonization antigens of enterotoxigenicEscherichia coli strains isolated from piglets in Spain. Vet.Microbiol. 1997;54:321–328. doi: 10.1016/S0378-1135(96)01291-6. PubMed DOI
Hacker J. Role of fimbrial adhesins in the pathogenesis ofEscherichia coli infections. Can.J.Microbiol. 1993;38:720–727. doi: 10.1139/m92-118. PubMed DOI
Hampson D.J. PostweaningE. coli in pigs. In: Gyles C.E., editor. Escherichia coliin Domestic Animals and Humans. Wallingford (UK): CAB International; 1994. pp. 178–181.
Holoda E., Mikula I. Construction of recombinant K88 DNA with Ptac promoter. Folia Microbiol. 1994;39:171–175. doi: 10.1007/BF02814643. PubMed DOI
Holoda E., Bianchi A.T.J., Smits M., Degeus B., Bergen H., Briare J., Mikula I.: Utilization of recombinant DNA technologies in immunoprophylaxis, p. 20 inProc. 21st Congr. Czechoslovak Society for Microbiology, Hradec Králové (Czechia) 1998.
Holoda E., Pistl J., Pilpćtnec E. General Microbiology and Genetic of Microorganism. Košice (Slovakia): Vienala; 2001.
Holoda E., Hung Vu Khac, Bergen H., Holečková B., Wantrubová A., Andrašková S., Pilipčinec E. Primers for the detection ofEscherichia coli K88ab+ Folia Vet. 2002;46:119–122.
Holoda E., Vu-Khac H., Andraškova S., Chomová Z., Pilipčinec E.: PCR assay for detection of F4ab1, F4ab2, F4ac and F4ad fimbrial adhesins inE. coli strains.Folia Microbiol.49, in press (2004). PubMed
Isaacson R.E., Moon H.W., Schneider R.A. Distribution and virulence ofEscherichia coli in the small intestines of calves with and without diarrhea. Am.J.Vet.Res. 1978;39:1750–1755. PubMed
Kaper J.B., Levine M.M. Progress towards a vaccine against enterotoxigenicE. coli. Vaccine. 1988;6:197–199. doi: 10.1016/S0264-410X(88)80028-8. PubMed DOI
Kwon D., Kim O., Chae C. Prevalence of genotypes for fimbriae and enterotoxins and of O serogroups inEscherichia coli isolated from diarrheic piglets in Korea. J.Vet.Diagn.Invest. 1999;11:146–151. PubMed
Martins M.F., Martinez-Rossi N.M., Ferreira A., Brocchi M., Yano T., Castro A.F.P., Silveira W.D. Pathogenic characteristics ofEscherichia coli strains isolated from newborn piglets with diarrhea in Brazil. Vet.Microbiol. 2000;76:51–59. doi: 10.1016/S0378-1135(00)00223-6. PubMed DOI
Morris J.A., Thorn C.J., Sojka W.J. Evidence for two adhesive antigens on the K99 reference strainsEscherichia coli B41. J.Gen.Microbiol. 1980;118:107–113. PubMed
Mullaney C.D., Francis D.H., Willoghs J.A. Comparison of seroagglutination, ELISA, and indirect fluorescent antibody staining for the detection of K99, K88, and 987P pilus antigens ofEscherichia coli. J.Vet.Diagn.Invest. 1991;3:115–118. PubMed
Nagy B., Fekete P.Z. EnterotoxigenicEscherichia coli (ETEC) in farms animals. Vet.Res. 1999;30:259–284. PubMed
Ojeniyi B., Arhens P., Meyling A. Detection of fimbrial and toxin genes inEscherichia coli and their prevalence in piglets with diarrhea. The application of colony hybridization assay, polymerase chain reaction and phenotypic assays. J.Vet.Med.B. 1994;41:49–59. doi: 10.1111/j.1439-0450.1994.tb00205.x. PubMed DOI
Ondrašovič M., Ondrašovičová O., Vargová M., Kočišová A. Environmental Problems in Veterinary Practice. Košice (Slovakia): University of Veterinary Medicine; 1997.
Ørskov I., Ørskov F., Sojka W.J., Leach J.M. Simultaneous occurence ofE. coli B and L antigens in strains from diseased swine. Acta Pathol.Microbiol.Scand.Sect.B. 1961;53:404–422. PubMed
Osek J. Prevalence of virulence factors ofEscherichia coli strains isolated from diarrheic and healthy piglets after weaning. Vet.Microbiol. 1999;68:209–217. doi: 10.1016/S0378-1135(99)00109-1. PubMed DOI
Osek J., Svennerhom A.M. Determination of K88 antigens and enterotoxins ofEscherichia coli strains isolated from Polish piglets with diarrhea by the use of enzyme-linked immunosorbent assays. Vet.Microbiol. 1992;29:299–307. doi: 10.1016/0378-1135(91)90137-5. PubMed DOI
Osek J., Truszczynsky M. Occurrence of fimbriae and enterotoxins inEscherichia coli strains isolated from piglets in Poland. Comp.Immunol.Microbiol.Infect.Dis. 1992;15:285–292. doi: 10.1016/0147-9571(92)90008-F. PubMed DOI
Rademaker C.M.A., Martinez-Martinez L., Perea E.J., Jansze M., Fluit A.C., Glerum J.H., Verhoef J. Detection of enterovirulentEscherichia coli with diarrhea in Sevilla, southern Spain, with nonradioactive DNA probes. J.Med.Microbiol. 1993;38:87–89. doi: 10.1099/00222615-38-2-87. PubMed DOI
Soderlind O., Thafvelin B., Mollby R. Virulence factors inEscherichia coli strains isolated from Swedish piglets with diarrhea. J.Clin.Microbiol. 1988;26:879–884. PubMed PMC
Stacy-Philips S., Mecca J.J., Weiss J.B. Multiplex PCR assay and simple preparation methods for stool specimens detect enterotoxigenicEscherichia coli DNA during the course of infection. J.Clin.Microbiol. 1995;33:1054–1059. PubMed PMC
Thorn C.J., Bell M.M., Chasey D., Chesham J., Roeder P.L. Development of monoclonal antibody ELISA for simultaneous detection of bovine coronavirus, rotavirus serogroup A, andEscherichia coli K99 antigen in feces of calves. Am.J.Vet.Res. 1992;53:36–43. PubMed
Thorns C.J., Sojka M.G., Roeder P.L. Detection of fimbrial adhesins of ETEC using monoclonal antibody-based latex reagents. Vet.Res. 1989;125:91–92. PubMed
Vu-Khac H., Holoda E., Wantrubová A., Andrašková S., Chomová Z., Čurlík J., Majerčiak M., Pilipčinec E. Using for PCR detection of F18 fimbrial antigen inE. coli strains isolated from piglets with diarrhea in Slovakia. Folia Vet. 2003;47:102–106.
Westermen R.B., Mills K.M., Pillips R.M., Fortner G.W., Greenwood J.M. Predominance of the ac variant in K88-positiveEscherichia coli isolated from swine. J.Clin.Microbiol. 1988;26:149–150. PubMed PMC
Wilson R.D., Francis D.H. Fimbriae and enterotoxin associated withEscherichia coli serogroups isolated from pigs with colibacillosis. Am.J.Vet.Res. 1986;47:213–217. PubMed
Wray C., McLaren I.M., Caroll P.J. Escherichia coli isolated from farm animals in England and Wales between 1986 and 1991. Vet.Rec. 1993;133:439–442. PubMed
Zhu C., Harel J., Jaques M., Desautels C., Donnenberg M.S., Beadry M., Fairbrother J.M. Virulence properties and attaching-effacing activityEscherichia coli O45 from swine postweaning diarrhea. Infect.Immun. 1994;62:4153–4159. PubMed PMC
Effect of lactobacilli on E. coli adhesion to Caco-2 cells in vitro
Curli expression of enterotoxigenic Escherichia coli