Inhibitory effect of various lactobacilli against pathogenic strains of E. coli in model system Caco2 cells was determined by enumerating the number of adhering E. coli after pre-incubation (exclusion), post-incubation (displacement) or co-incubation (competition) with lactobacilli. Porcine E. coli strain F107 (F18ab, Stx2v) in the competition assay with porcine lactobacillus strain P10 gave bacterial counts 7.25 (log CFU per well); in the exclusion test it was only 7.05 while in displacement test it reached 7.29. The lowest E. coli counts adhering to Caco-2 cells were in exclusion assay (pre-incubation, Lactobacillus inoculated as the first). Pre-treatment of E. coli with our lactobacilli strains reduced the cultivable E. coli numbers.

Institute of Animal Physiology Slovak Academy of Sciences Kosice Slovakia

Zobrazit více v PubMed

J Gen Microbiol. 1992 Aug;138 Pt 8:1689-96 PubMed

Folia Microbiol (Praha). 2005;50(5):443-7 PubMed

Zentralbl Veterinarmed B. 1999 Dec;46(10):683-7 PubMed

Appl Environ Microbiol. 1992 Jun;58(6):2034-9 PubMed

Int J Food Microbiol. 1998 May 5;41(1):45-51 PubMed

Folia Microbiol (Praha). 2005;50(2):107-12 PubMed

Folia Microbiol (Praha). 2004;49(1):59-63 PubMed

Can J Microbiol. 1988 Aug;34(8):974-8 PubMed

Folia Microbiol (Praha). 2004;49(2):203-7 PubMed

Folia Microbiol (Praha). 2004;49(2):143-6 PubMed

J Vet Med B Infect Dis Vet Public Health. 2002 Apr;49(3):152-4 PubMed

Appl Environ Microbiol. 1993 Dec;59(12):4121-8 PubMed

Bile tolerance and its effect on antibiotic susceptibility of probiotic Lactobacillus candidates

Inhibitory potential of lactobacilli against Escherichia coli internalization by HT 29 cells

Auto-aggregation and co-aggregation ability in bifidobacteria and clostridia

The protective potency of probiotic bacteria and their microbial products against enteric infections-review

Probiotic potential of enterococci isolated from canine feed

Impact of colostomy on intestinal microflora and bacterial translocation in young rats fed with heat-killed Lactobacillus acidophilus strain LB

The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation

Adaptation of adhesion test using Caco-2 cells for anaerobic bacterium Pseudobutyrivibrio xylanivorans, a probiotic candidate

Analysis of the presence of prtR proteinase gene in natural isolates of Lactobacillus rhamnosus