Curli expression of enterotoxigenic Escherichia coli
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15954532
DOI
10.1007/bf02931292
Knihovny.cz E-resources
- MeSH
- Bacterial Adhesion MeSH
- Fimbriae, Bacterial * ultrastructure MeSH
- Genes, Bacterial MeSH
- Bacterial Proteins analysis MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Enterotoxins biosynthesis MeSH
- Escherichia coli genetics isolation & purification ultrastructure MeSH
- Congo Red metabolism MeSH
- Escherichia coli Proteins chemistry genetics immunology MeSH
- Gene Expression Regulation, Bacterial MeSH
- Genes, Regulator MeSH
- Temperature MeSH
- Microscopy, Electron, Transmission MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Crl protein, Bacteria MeSH Browser
- csgA protein, E coli MeSH Browser
- Enterotoxins MeSH
- Congo Red MeSH
- Escherichia coli Proteins MeSH
One hundred and four enterotoxin producing Escherichia coli strains of wide geographical origin were tested for the expression of curli fimbriae by transmission electronmicroscopy and by ELISA using curli-specific antibodies, as well as for the presence of curli-specific gene sequences by PCR. All isolates, irrespective of the production of the fimbriae, carried sequences specific for the structure (csgA) and for one of the regulator genes (crl) of curli expression, respectively. Curli fimbriae were detected in 56 strains (53.8 %). Thirty-six strains expressed curli only when growing at 30 degrees C, 4 isolates were weakly curliated at 37 degrees C only, while on 16 strains curli was observed at both temperatures. On isolates carrying curli at both temperatures the expression of the fimbria was significantly stronger at 30 degrees C than at 37 degrees C. Curli proficiency significantly, but not completely, correlated with the binding of the Congo Red dye. The expression of curli did not confer epithelial cell invasiveness to ETEC strains but, once expressed at 30 degrees C, it facilitated the adherence of the bacteria to plastic surfaces. Curli present in more than half of the ETEC strains and expressed preferentially at low temperatures could be a factor facilitating the environmental survival of this food- and water-borne pathogen.
See more in PubMed
J Bacteriol. 2003 Sep;185(18):5398-407 PubMed
Infect Immun. 1997 Dec;65(12):5320-5 PubMed
FEMS Microbiol Lett. 1998 May 15;162(2):295-301 PubMed
Mol Microbiol. 1992 Sep;6(17):2443-52 PubMed
Appl Environ Microbiol. 2001 May;67(5):2367-70 PubMed
Int J Med Microbiol. 2002 Sep;292(3-4):195-205 PubMed
Infect Immun. 1992 Jun;60(6):2409-17 PubMed
J Med Microbiol. 2000 Apr;49(4):327-338 PubMed
J Bacteriol. 1998 May;180(9):2442-9 PubMed
J Clin Microbiol. 1985 Dec;22(6):996-1006 PubMed
Folia Microbiol (Praha). 2002;47(2):185-8 PubMed
Nat Med. 1998 Mar;4(3):298-302 PubMed
Folia Microbiol (Praha). 2003;48(6):823-8 PubMed
Mol Microbiol. 1995 Nov;18(4):661-70 PubMed
J Bacteriol. 1993 Jan;175(1):12-8 PubMed
J Bacteriol. 1991 Aug;173(15):4773-81 PubMed
J Bacteriol. 1992 Jul;174(13):4490-5 PubMed
Clin Microbiol Rev. 1998 Jan;11(1):142-201 PubMed
Mol Microbiol. 1994 Nov;14(3):443-52 PubMed
Infect Immun. 1977 Nov;18(2):330-7 PubMed
Mol Microbiol. 2001 Mar;39(6):1452-63 PubMed
Avian Dis. 1998 Jan-Mar;42(1):106-18 PubMed
Adv Exp Med Biol. 2000;485:219-24 PubMed
Zentralbl Veterinarmed B. 1988 Dec;35(10):779-82 PubMed
Appl Microbiol. 1969 Nov;18(5):834-7 PubMed
Microbiology (Reading). 1999 May;145 ( Pt 5):1023-1031 PubMed
J Infect Dis. 2000 Feb;181(2):602-12 PubMed
Nature. 1989 Apr 20;338(6217):652-5 PubMed
Infect Immun. 2000 Jun;68(6):3780-3 PubMed
Folia Microbiol (Praha). 2003;48(2):243-7 PubMed
Infect Immun. 1998 Mar;66(3):944-9 PubMed
Infect Immun. 1984 Jan;43(1):397-401 PubMed
J Clin Microbiol. 1995 May;33(5):1054-9 PubMed
Infect Immun. 2001 Apr;69(4):2659-65 PubMed
Folia Microbiol (Praha). 2003;48(5):671-8 PubMed
Infect Immun. 2003 Jul;71(7):4151-8 PubMed
Folia Microbiol (Praha). 2004;49(1):59-63 PubMed
J Infect Dis. 2001 Feb 15;183(4):612-9 PubMed
Infect Immun. 1992 Nov;60(11):4460-7 PubMed
Mol Microbiol. 2003 May;48(4):933-46 PubMed
Mol Microbiol. 1996 Jun;20(5):927-35 PubMed
Infect Immun. 2002 Jan;70(1):395-9 PubMed
Mol Microbiol. 1993 Feb;7(4):523-36 PubMed
Mol Microbiol. 1998 Apr;28(2):249-64 PubMed
Mol Microbiol. 1994 Sep;13(6):1021-32 PubMed
Diversity of fliC gene in commensal Escherichia coli derived from various mammals
