Occurrence of extended-spectrum beta-lactamases among Escherichia coli isolates from hospitalized and healthy children
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
12800510
DOI
10.1007/bf02930963
Knihovny.cz E-zdroje
- MeSH
- beta-laktamasy metabolismus MeSH
- beta-laktamová rezistence MeSH
- beta-laktamy farmakologie MeSH
- dítě MeSH
- Escherichia coli klasifikace enzymologie izolace a purifikace MeSH
- hospitalizace * MeSH
- infekce vyvolané Escherichia coli epidemiologie mikrobiologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- předškolní dítě MeSH
- průjem epidemiologie mikrobiologie MeSH
- střevní sliznice mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- předškolní dítě MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- beta-laktamasy MeSH
- beta-laktamy MeSH
The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-positive isolate came from a healthy child. The transfer frequency of oxyimino-beta-lactam resistance from ESBL-producing isolates to E. coli K12 C600 recipient strain ranged from 10(-8) to 10(-5) per donor cell. Donor strains and transconjugants displayed susceptibility patterns typical of ESBL producers. They were resistant to oxyimino-beta-lactams but susceptible to clavulanic acid and carbapenems. Seven out of the 10 ESBL-positive isolates were found to produce MR/MS fimbria, which may play an important role in the colonization of the human intestinal mucosa.
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