Twenty (18.5%) out of 108 clinical isolates of the family Enterobacteriaceae responsible for bloodstream infection were extended-spectrum beta-lactamase (ESBL)-positive in two screening tests, the double disk synergy test and the Oxoid Combination Disk method. Eleven out of the 20 ESBL-positive isolates transferred oxyimino-beta-lactam resistance to E. coli K12 C600 recipient strain with a frequency of 10(-8) - 10(-1) per donor cell. PCR analysis revealed that the majority of the transconjugants (9 of 11) express CTX-M-type beta-lactamases. Donor strains and their transconjugants displayed susceptibility patterns typical of ESBL producers. They were resistant to oxyimino-beta-lactams but susceptible to clavulanic acid and carbapenems. Resistances to aminoglycosides, tetracycline and mercuric chloride were, in some cases, co-transferred with oxyimino-beta-lactam resistance, suggesting that various resistance determinants were carried by the same conjugative plasmids.

Department of Microbiology University of Medicine in Wrocław 50 368 Wrocław Poland

Zobrazit více v PubMed

Antimicrob Agents Chemother. 1998 Apr;42(4):827-32 PubMed

JAMA. 1995 Aug 23-30;274(8):639-44 PubMed

Clin Infect Dis. 1997 Jan;24 Suppl 1:S19-45 PubMed

Antimicrob Agents Chemother. 2004 Jan;48(1):1-14 PubMed

J Bacteriol. 1977 Jan;129(1):276-81 PubMed

Clin Microbiol Rev. 1998 Oct;11(4):589-603 PubMed

Antimicrob Agents Chemother. 1991 Sep;35(9):1697-704 PubMed

Chest. 1991 Jul;100(1):164-7 PubMed

Antimicrob Agents Chemother. 1998 Dec;42(12):3079-85 PubMed

Folia Microbiol (Praha). 2003;48(2):243-7 PubMed

Zentralbl Bakteriol. 1997 Nov;286(4):449-55 PubMed

J Antimicrob Chemother. 2003 Feb;51(2):470-1 PubMed

Infection. 1983 Nov-Dec;11(6):315-7 PubMed

Antimicrob Agents Chemother. 1991 Jan;35(1):164-9 PubMed

Antimicrob Agents Chemother. 2001 Aug;45(8):2269-75 PubMed

Eur J Clin Microbiol Infect Dis. 1994;13 Suppl 1:S2-11 PubMed

Arch Intern Med. 1995 Jun 12;155(11):1177-84 PubMed

Antimicrob Agents Chemother. 2004 Apr;48(4):1249-55 PubMed

J Antimicrob Chemother. 1996 Sep;38(3):409-24 PubMed

Antimicrob Agents Chemother. 2001 Dec;45(12):3355-61 PubMed

Folia Microbiol (Praha). 2004;49(6):763-8 PubMed

Plasmid. 1990 Jan;23(1):27-34 PubMed

Int J Antimicrob Agents. 2000 Mar;14(2):137-42 PubMed

Clin Microbiol Rev. 2001 Oct;14(4):933-51, table of contents PubMed

J Clin Microbiol. 1999 Dec;37(12):4020-7 PubMed

J Antimicrob Chemother. 1993 Oct;32(4):559-70 PubMed

Infect Control Hosp Epidemiol. 2000 Aug;21(8):510-5 PubMed

J Clin Microbiol. 2000 Nov;38(11):4228-32 PubMed

Folia Microbiol (Praha). 2004;49(4):457-64 PubMed

Clin Microbiol Rev. 1995 Oct;8(4):557-84 PubMed

Folia Microbiol (Praha). 2001;46(4):339-44 PubMed

Rev Infect Dis. 1988 Jul-Aug;10(4):867-78 PubMed

Molecular epidemiology of PER-1 extended spectrum beta-lactamase among gram-negative bacteria isolated at a tertiary care hospital

Seasonal dynamics of antibiotic-resistant Enterobacteriaceae in the gastrointestinal tract of domestic sheep