Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články
- MeSH
- bakteriální proteiny analýza MeSH
- bakteriologické techniky metody MeSH
- DNA bakterií chemie genetika MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- kozy MeSH
- Limosilactobacillus reuteri klasifikace genetika izolace a purifikace fyziologie MeSH
- mezerníky ribozomální DNA chemie genetika MeSH
- ovce MeSH
- polymerázová řetězová reakce MeSH
- reprodukovatelnost výsledků MeSH
- shluková analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- žaludeční sliznice mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- srovnávací studie MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- mezerníky ribozomální DNA MeSH
Several biochemical and molecular methods were used for discrimination of four Lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. Internal transcribed spacer (ITS)-PCR method and protein analysis by SDS-PAGE and MALDI-TOF showed to be suitable for strain discrimination whereas ITS-PCR/RFLP and enterobacterial repetitive intergenic consensus (ERIC)-PCR were not strain specific. The used methods differentiated tested strains into distinct groups; however, the location of strains in groups varied. Consistency in results was observed in the case of L. reuteri E and L. reuteri KO4m that were clustered into the same groups using all techniques, except of MALDI-TOF MS. The last one grouped goatling strains and lamb isolate into separate clusters. All investigated methods, except of ITS-PCR/RFLP and ERIC-PCR, were assessed as appropriate for distinguishing of L. reuteri strains.
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