Isolation and identification of new lactobacilli from goatling stomach and investigation of reuterin production in Lactobacillus reuteri strains
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- glyceraldehyd analogy a deriváty metabolismus MeSH
- kozy mikrobiologie MeSH
- Lactobacillus izolace a purifikace MeSH
- Limosilactobacillus reuteri metabolismus MeSH
- propan metabolismus MeSH
- žaludek mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 3-hydroxypropionaldehyde MeSH Prohlížeč
- glyceraldehyd MeSH
- propan MeSH
Five new strains of lactobacilli isolated from goatling's stomach were identified by molecular-biological approaches. Profiles of fermentable saccharides, Gram staining, and cell morphology were also determined. They were identified as Lactobacillus reuteri (strains KO4b, KO4m, KO5) and as Lactobacillus plantarum (strains KG1z, KG4). In DNA samples of all newly isolated L. reuteri strains as well as in L. reuteri E (Lreu E; originated from lamb), the part of gldC gene, coding large subunit of glycerol dehydratase, that is necessary for 3-hydroxypropionaldehyde (3-HPA; reuterin) production, was amplified using two designed primer sets. However, the 3-HPA production was revealed only in the strain Lreu E. It produced five- or ten-fold lower amount of 3-HPA in comparison with probiotic L. reuteri ATCC 55730 in aerobic or anaerobic conditions, respectively. Moreover, Lreu E completely lost its production ability after ca. five passages in MRS medium. The co-incubation of Lreu E, but not other L. reuteri isolates, with Escherichia coli re-induced 3-HPA production. In the case of L. reuteri ATCC 55730, the 3-HPA production increased more than four times after co-incubation with E. coli.
Zobrazit více v PubMed
FEMS Microbiol Lett. 2004 Oct 15;239(2):267-75 PubMed
New Microbiol. 2005 Jul;28(3):265-70 PubMed
Appl Microbiol Biotechnol. 2004 Mar;64(1):16-27 PubMed
Int J Food Microbiol. 2005 Oct 25;104(3):267-77 PubMed
Int J Food Microbiol. 2000 Apr 10;55(1-3):167-70 PubMed
Appl Environ Microbiol. 2008 Aug;74(15):4645-9 PubMed
PLoS One. 2011 Apr 29;6(4):e18783 PubMed
Antimicrob Agents Chemother. 1988 Dec;32(12):1854-8 PubMed
Appl Environ Microbiol. 2002 Jan;68(1):114-23 PubMed
J Bacteriol. 2003 Sep;185(18):5643-7 PubMed
Antimicrob Agents Chemother. 1989 May;33(5):674-9 PubMed
DNA Res. 2008 Jun 30;15(3):151-61 PubMed
FEBS Lett. 2007 Oct 16;581(25):4865-70 PubMed
Microb Cell Fact. 2011 Jul 21;10:55 PubMed
J Clin Microbiol. 2007 Sep;45(9):2761-4 PubMed
Anaerobe. 2008 Jun;14(3):166-71 PubMed
J Appl Microbiol. 1997 Jun;82(6):783-90 PubMed
J Bacteriol. 2008 Jul;190(13):4559-67 PubMed
Microbiology (Reading). 2010 Jun;156(Pt 6):1589-1599 PubMed
Braz J Microbiol. 2011 Jul;42(3):1188-96 PubMed
FEMS Microbiol Lett. 1998 Apr 1;161(1):97-106 PubMed
Appl Microbiol Biotechnol. 2002 Oct;60(1-2):73-80 PubMed
J Clin Microbiol. 2000 Oct;38(10):3623-30 PubMed
Bile tolerance and its effect on antibiotic susceptibility of probiotic Lactobacillus candidates
Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation
